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Nucleic Acids Research, 1992, Vol. 20, No. 16 4221-4227
© 1992

MOLECULAR BIOLOGY

A ribosomal ambiguity mulation in the 530 loop of E.coli 16S rRNA

Michael O'Connor*, H. Ulrich Göringer1,+ and Albert E. Dahiberg

Section of Biochemistry, Box G, Brown University Providence, RI 02912, USA 1Max-Planck Institute für Molekulare Genetik Ihnestrasse 73, 1000 Berlin 33, Germany

*To whom correspondence should be addressed

Received May 26, 1992. Revised July 20, 1992. Accepted July 20, 1992.

A series of base substitution and deletion mutations were constructed in the highly conserved 530 stem and loop region of E.coli 16S rRNA involved in binding of tRNA to the ribosomal A site. Base substitution and deletion of G517 produced significant effects on cell growth rate and translational fidelity, permitting readthrough of UGA, UAG and UAA stop codons as well as stimulating +1 and –1 frameshlftlng in vivo. By contrast, mutations at position 534 had little or no effect on growth rate or translational fidelity. The results demonstrate the Importance of G517 in maintaining translational fidelity but do not support a base pairing Interaction between G517 and U534.

+Present address: Laboratorium für Molekular Biologie, Gezentrum der Ludwig-Maximilian-Universität München, Arn Klopferspitz 18, 8033 Martinsried, Germany


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