Nucleic Acids Research, 1992, Vol. 20, No. 16 4311-4318
© 1992
MOLECULAR BIOLOGY |
Multiple ocatamer binding sites in the promoter region of the bovine
s2-Casein gene
Department of Animal Breeding, Agricultural University Wageningen PO Box 338, 6700 AH Wageningen, The Netherlands
Received April 15, 1992. Revised July 20, 1992.
Using a set of overlapping oligonucleotides from the promoter region of the bovine 
s2-casein gene we have identified two nuclear factors which probably are involved In expression of this gene and the related calcium sensitive s1- and ß-caseln genes. One of these factors which was present In extracts of all tissues that have been tested including Hela cells turned out to be the octamer binding protein OCT-1. Oct-1 binds with different affinity to 4 sites at positions centred around -480, -260, -210 and -50. The strongest of these 4 binding shies, the one around position -50, is highly conserved in all calcium sensitive caseins of mouse, rat, rabbit and cattle. The other nuclear factor (MGF, mammary gland factor) which is specifically expressed In the mammary gland, binds to a site around position - 90. This binding site is also highly conserved in all calcium sensitive caseins of mouse, rat, rabbit and cattle.
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