Skip Navigation

This Article
Right arrow Print PDF (478K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (46)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by de Biologia, R. C.
Right arrow Articles by Menck, C. F.M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de Biologia, R. C.
Right arrow Articles by Menck, C. F.M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 16 4319-4323
© 1992

MOLECULAR BIOLOGY

Singlet Oxygen induced mutation spectrum in mammalian cells

Reginas Costa de Biologia, Denise T. Riberio, Rogerio G. Nigro, Paolo Di Mascio+ and Carlos F.M. Menck*

Departamento de Biologia, Instituto de Biochiencia, Universidade de Sao Paulo CP 11461, Sao Paulo 05499, Brazil

*To whom correspondence should be addressed

Received March 23, 1992. Revised July 18, 1992.

In order to characterize the molecular nature of singlet oxygen (1O2) induced mutations In mammalian cells, a SV40-based shuttle vector ({pi}SVPC13) was treated with singlet oxygen arising from the thermal decomposition of the water-soluble endoperoxide of 3,3'-(1,4-naphthytidene) dipropionate (NDPOJ- After the passage of damaged plasmld through monkey COS7 cells, the vector was shuttled Into E.coll cells, allowing the screening of supF mutants. The mutation spectrum analysis shows that single and multiple base substitutions arose in 82.5% of the mutants, the others being rearrangements. The distribution of mutations within the supF gene Is not random and some hotspots are evident. Most of the point mutations (98.4%) involve G:C base pairs and G:C to T:A transversion was the most frequent mutation (50.8%), followed by G:C to C:G transversion (32.8%). These results indicate that mutagenesis in mammalian cells, mediated by 1O2-induced DNA damage, is targeted selectively at guanine esidues.

+Present address: Instituto de Qufmica, CP 20780, USP, Sao Paulo, 05499 SP. Brazil


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
T. K. Hazra, T. Izumi, I. Boldogh, B. Imhoff, Y. W. Kow, P. Jaruga, M. Dizdaroglu, and S. Mitra
From the Cover: Identification and characterization of a human DNA glycosylase for repair of modified bases in oxidatively damaged DNA
PNAS, March 19, 2002; 99(6): 3523 - 3528.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. F. Agnez-Lima, R. L. Napolitano, R. P. P. Fuchs, P. D. Mascio, A. R. Muotri, and C. F. M. Menck
DNA repair and sequence context affect 1O2-induced mutagenesis in bacteria
Nucleic Acids Res., July 1, 2001; 29(13): 2899 - 2903.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. K. Hazra, J. G. Muller, R. C. Manuel, C. J. Burrows, R. S. Lloyd, and S. Mitra
Repair of hydantoins, one electron oxidation product of 8-oxoguanine, by DNA glycosylases of Escherichia coli
Nucleic Acids Res., May 1, 2001; 29(9): 1967 - 1974.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
B.S. Shane, J.G. deBoer, B.W. Glickman, and M.L. Cunningham
Oxazepam is mutagenic in vivo in Big Blue(R) transgenic mice
Carcinogenesis, July 1, 1999; 20(7): 1315 - 1321.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.