Skip Navigation

This Article
Right arrow Print PDF (4784K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kaufmann, J.
Right arrow Articles by Klein, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kaufmann, J.
Right arrow Articles by Klein, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 17 4445-4450
© 1992

MOLECULAR BIOLOGY

Gene dosage as a possible major determinant for equal expression levels of genes encoding RNA polymerase subunits in the hypotrichous ciliate Euplotes octocarinatus

Jörg Kaufmann and Albrecht Klein*

Molecular Genetics, Department of Biology, Philipps University PO Box 1929, W-3550 Marburg, Germany

*To whom correspondence should be addressed

Received June 17, 1992. Revised August 3, 1992. Accepted August 3, 1992.

Ciliated protozoa harbor two different types of nuclei in each cell. The diploid micronucleus is the transcriptionally inactive generative nucleus, while the macronuclous contains a highly amplified transcriptionally active genome of lower complexity. The macronuclear genes encoding the two largest subunits of both RNA polymerases I and II of Euplotes octocarinatus were identified by a novel method of two step PCR walking, employing primer pairs derived from telomeric sequences of the organism and known conserved RNA polymerase polypeptide sequences, respectively. The relative gene dosage was determined. The genes are present in equal copy numbers for the respective matching subunits. Northern hybridizations showed comparable amounts of transcripts, as well, within the matching pairs. Mapping of the 5'-termini of the transcripts of the gene sized chromosomes showed that the upstream nontranscribed regions are very short and contain characteristic sequence motifs which could be the determinants of equal promoter strengths for subunits of a common RNA polymerase.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Eukaryot CellHome page
I. Skovorodkin, A. Pimenov, I. Raykhel, B. Schimanski, D. Ammermann, and A. Gunzl
{alpha}-Tubulin Minichromosome Promoters in the Stichotrichous Ciliate Stylonychia lemnae
Eukaryot. Cell, January 1, 2007; 6(1): 28 - 36.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. M. Prescott and S. J. Dizick
A unique pattern of intrastrand anomalies in base composition of the DNA in hypotrichs
Nucleic Acids Res., December 1, 2000; 28(23): 4679 - 4688.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.