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Nucleic Acids Research, 1992, Vol. 20, No. 17 4451-4455
© 1992

MOLECULAR BIOLOGY

Stoichiometry of the Cre recombinase bound to the lox recombining site

Alison Mack, Brian Sauer, Kenneth Abremski and Ronald Hoess*

The DuPont Merck Pharmaceutical Company, Experimental Station Box 80328, Wilmington, DE 19880-0328, USA

*To whom correspondence should be addressed

Received June 16, 1992. Accepted July 29, 1992.

The site-specific recombinase Cre from bacteriophage P1 binds and carries out recombinatlon at a 34 bp lox site. The lox site consists of two 13 bp inverted repeats, separated by an 8 bp spacer region. Both the palindromic nature of the site and the results of footprinting and band shift experiments suggest that a minimum of two Cre molecules bind to a lox site. We report here experiments that demonstrate the absolute stoichiometry of the Cre-lox complex to be one molecule of Cre bound per Inverted repeat, or two molecules per lox site.


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