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Nucleic Acids Research, 1992, Vol. 20, No. 17 4499-4505
© 1992

MOLECULAR BIOLOGY

Thiolation of transfer RNA in Escherichia coli varies with growth rate

Valur Emilsson, Anna K. Näslund and Charles G. Kurlad

Department of Molecular Biology, Uppsala University, Biomedical Center Box 590, S-751 24, Uppsala, Sweden

Received May 28, 1992. Revised August 14, 1992. Accepted August 14, 1992.

We have used an affinity electrophoresis assay which when combined with Northern hybridization techniques permits us to estimate the degree of thiolation of individual tRNA species in Escherichia coli. We observe that the levels of 4-thio 2'(3')-uridine (4-thioU) in many but not all tRNAs varies dramatically at different bacterial growth rates: Five tRNAs are completely thiolated at all growth rates, while another eight tRNAs are incompletely thiolated and the fraction of the unthiolated form of these tRNA species increases as the growth rates increase. Transfer Formula contains 4-thioU as well as (methylamino)methyl-2-thio uridine (mnm52-thioU). The level of mnm52-thioU of Formula is invariant with growth rate. Surprisingly, none of the thirteen tRNA species that we have studied is completely unmodified in all growth media. In particular, at the slowest growth rates every tRNA class that we have studied contains a form that has 4-thioU residues.


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