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Nucleic Acids Research, 1992, Vol. 20, No. 18 4747-4751
© 1992

STRUCTURAL BIOLOGY

Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants

P. Schmieder, J.H. Ippel1, H.van den Elst1, G.A.van der Marel1, J.H.van Boom1, C. Altona1 and H. Kessler*

Organisch-Chemisches Institut der TU München Lichtenbergstraße 4, D-8046 Garching, Germany 1Department of Organic Chemistry, Gorlaeus Laboratories, Leiden University PO Box 9502, 2300 RA Leiden, The Netherlands

*To whom correspondence should be addressed

Received July 6, 1992. Revised August 17, 1992. Accepted August 17, 1992.

Two heteronuclear proton-carbon NMR experiments are applied to the DNA-octamer d(TTGGCCAA)2 with carbon in natural abundance. They lead to a complete assignment of the carbon resonances of the sugars and bases. In addition, several heteronuclear coupling constants, proton-carbon as well as proton-phosphorous and phosphorous-carbon, were determined. The information can be obtained in a resonable measuring time and offers valuable information for a detailed picture of DNA structure.


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