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Nucleic Acids Research, 1992, Vol. 20, No. 18 4865-4872
© 1992

MOLECULAR BIOLOGY

Activation and repression sequences determine the lens-specific expression of the rat {gamma}D-crystallin gene

Ron Peek, Harry J. Kraft, Erik J. Klok, Nicolette H. Lubsen and John G.G. Schoenmakers*

Department of Molecular Biology, University of Nijmegen, Toernooiveld 6525 ED Nijmegen, The Netherlands

*To whom correspondence should be addressed

Received June 5, 1992. Revised August 28, 1992. Accepted August 28, 1992.

Rat lens nuclear extracts contain a factor that binds to position -57 to -46 of the rat {gamma}D-crystallin promoter region. This factor protects the sequence 5'-CTGCCAA- CGCAG-3' in a footprint analysis. Binding to this region Is crucial for maximal promoter activity In rat lens cells, but this sequence was unable to act as an enhancer when cloned in front of a heterologous promoter. A region directly upstream from this activating sequence, between posItion -85 to -67, acts as a strong silencer of promoter activity in non-lens cells. This silencing effect is mediated by trans-acting factor(s). Our data provide evidence for two regulatory elements In rat {gamma}D-crystallin gene expression, an activating sequence active in lens cells and a silencing sequence active only In non-lens cells. The factor that binds to the activating sequence could be detected only in lens cells and may be a determinant of the lens-specific expression of the {gamma}-crystallin genes.


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