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Nucleic Acids Research, 1992, Vol. 20, No. 18 4913-4918
© 1992


MOLECULAR BIOLOGY

The 66 kDa component of yeast SF1, stimulatory factor I, is hsp6O

Jean K. Smiley, William Clay Brown and Judith L. Campbell*

Braun Laboratories, Division of Chemistry California Institute of Technology, Pasadena, CA 91125, USA

1To whom correspondence should be addressed

Received April 29, 1992. Revised August 12, 1992. Accepted August 12, 1992.

DNA polymerase e stimulatory factor I (SFI) has been shown to contain three peptldes, p66, p37 and p13 Two of these components have been identified The p66 gene was cloned by using a p66 antibody to screen a {lambda}gt11 library.A portion of the gene was sequenced and confirmed to encode p66 by the presence of protein sequence corresponding to that of p66 tryptic peptides.The gene was identified as HSP60 by a homology search of GenBank Tryptic peptides of p37 were sequenced and identified as belonging to yeast translation initiation factor 4A by a homology search of PIR The HSP6O gene maps to chromosome XII.


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