Skip Navigation

This Article
Right arrow Print PDF (447K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (54)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kierzek, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kierzek, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 19 5073-5077
© 1992

CHEMISTRY

Hydrolysis of oligoribonucleotides: influence of sequence and length

Ryszard Kierzek*

Department of Chemistry, University of Rochester Rochester, NY 14627-0216, USA Institute of Bioorganic Chemistry, Polish Academy of Sciences 60-704 Poznan, Noskowskiego 12/14, Poland

* Corresponding address: Institute of Bioorganic Chemistry, Polish Academy of Sciences, 60-704 Poznan, Noskowskiego 12/14, Poland

Received July 9, 1992. Revised September 7, 1992. Accepted September 7, 1992.

The chemical stability of phosphodiester bonds of some oligoribonucleotides in the presence of a cofactor like polyvinylpyrolidine (PVP) is sequence dependent. It was found that pyrimidine-A (YA) and pyrimidine-C (YC) are especially susceptible to hydrolysis. The hydrolyzability of this same phosphodiester bond is dependent on its position in the oligomer. The presence of 3' and 5'-adjacent nucleotides enhances hydrolysis of the UA phosphodiester bond. The acceleration of the hydrolysis of UA by a 5'-adjacent nucleotide is not base dependent. However, a 3'-adjacent purine increases hydrolysis of a UA phosphodiester bond more than a 3'-pyrimidine. The presence of the exoamlno group on the 3'-side base (on 6 and 4 position for adenosine and cytldlne, respectively) of YA or YZ phosphodiester bond is required for hydrolysis.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
I. L. Kuznetsova, M. A. Zenkova, H. J. Gross, and V. V. Vlassov
Enhanced RNA cleavage within bulge-loops by an artificial ribonuclease
Nucleic Acids Res., February 24, 2005; 33(4): 1201 - 1212.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Sakamoto, T. Tamura, T. Furukawa, Y. Komatsu, E. Ohtsuka, M. Kitamura, and H. Inoue
Highly efficient catalytic RNA cleavage by the cooperative action of two Cu(II) complexes embodied within an antisense oligonucleotide
Nucleic Acids Res., March 1, 2003; 31(5): 1416 - 1425.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
U. Kaukinen, S. Lyytikainen, S. Mikkola, and H. Lonnberg
The reactivity of phosphodiester bonds within linear single-stranded oligoribonucleotides is strongly dependent on the base sequence
Nucleic Acids Res., January 15, 2002; 30(2): 468 - 474.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
W. C. Putnam, A. T. Daniher, B. N. Trawick, and J. K. Bashkin
Efficient new ribozyme mimics: direct mapping of molecular design principles from small molecules to macromolecular, biomimetic catalysts
Nucleic Acids Res., May 15, 2001; 29(10): 2199 - 2204.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Figlerowicz
Role of RNA structure in non-homologous recombination between genomic molecules of brome mosaic virus
Nucleic Acids Res., April 15, 2000; 28(8): 1714 - 1723.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Bibillo, M. Figlerowicz, and R. Kierzek
The non-enzymatic hydrolysis of oligoribonucleotides VI. The role of biogenic polyamines
Nucleic Acids Res., October 1, 1999; 27(19): 3931 - 3937.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.