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Nucleic Acids Research, 1992, Vol. 20, No. 19 5079-5084
© 1992

CHEMISTRY

Nonenzymatic hydrolysis of oligoribonucleotides

Ryszard Kierzek*

Department of Chemistry, University of Rochester Rochester, NY 14627-0216, USA Institute of Bioorganic Chemistry, Polish Academy of Sciences 60-704 Poznan, Noskowskiego 12/14, Poland

* Corresponding address: Institute of Bioorganic Chemistry, Polish Academy of Sciences, 60-704 Poznan, Noskowskiego 12/14, Poland

Received July 9, 1992. Revised September 7, 1992. Accepted September 7, 1992.

Selective cleavage of phosphodlester bonds in RNA is important in the processing of large RNA molecules. This paper reports specific cleavage at UA sequences in single stranded oligoribonucleotides as short as hexamers. The hydrolysis between U and A leaves a 2',3'-cyclic phosphate on the 5'-side and a 5'-hydroxyl group on the 3' side of the cleavage. The hydrolysis is promoted by a wide range of cofactors, including polymeric organic compounds such as polyvlnylpyrrolydone (PVP) and by proteins. A variety of experiments suggests the cleavage is not due to contamination by ribonuclease. The rate of cleavage is a function of oligoribonucleotlde, PVP and spermidine concentrations. Mg2+ is not required. The phenomenon described here can potentially provide a relatively simple way of coding chemical stability into single stranded RNA based on its sequence and structure. This process seems to be similar to that involved in post-transcriptional degradation of mRNA.


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