Nucleic Acids Research, 1992, Vol. 20, No. 19 5093-5096
© 1992
MOLECULAR BIOLOGY |
A novel POU family transcription factor is closely related to Brn-3 but has a distinct expression pattern in neuronal cells
1Medical Molecular Biology Unit, Departments of Biochemistry and Chemical Pathology, Division of Molecular Pathology, University College and Middlesex School of Medicine The Windeyer Building, Cleveland Street, London W1P 6DB, UK 2Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital Du Cane Road, London W12 OHS, UK 3Medical Molecular Biology Unit, Departments of Biochemistry and Chemical Pathology, Division of Molecular Pathology, University College and Middlesex School of Medicine The Windeyer Building, Cleveland Street, London W1P 6DB, UK
* To whom correspondence should be addressed
Received June 29, 1992. Revised September 4, 1992. Accepted September 4, 1992.
The use of the polymerase chain reaction in conjunction with degenerate oligonucleotides has allowed the isolation of cDNA clones derived from each of the POU family transcription factors expressed in the proliferating ND7 neuronal cell line. In addition to the previously characterized Oct-1, Oct-2 and Brn-3 factors, ND7 cells have been shown by this means to express a novel POU factor. This factor is closely related to Brn-3 but differs at seven amlno acids in the POU domain, one of which is located in a region which is critical for protein-protein interactions between different POU proteins. Like Brn-3, the novel factor is expressed at high levels in embryonic brain and declines in abundance during neuronal development. In contrast to Brn-3 however, it is absent in mature sensory neurons and its expression declines during the in vitro differentiation of ND7 cells to a non-dividing phenotype whilst Brn-3 expression increases. The significance of these distinct but overlapping expression patterns is discussed in terms of the possible role of these two factors in regulating neuronal gene expression.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Peixoto, Y. Liu, S. Depauw, M.-P. Hildebrand, D. W. Boykin, C. Bailly, W. D. Wilson, and M.-H. David-Cordonnier Direct inhibition of the DNA-binding activity of POU transcription factors Pit-1 and Brn-3 by selective binding of a phenyl-furan-benzimidazole dication Nucleic Acids Res., June 1, 2008; 36(10): 3341 - 3353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. S. Budhram-Mahadeo, S. Bowen, S. Lee, C. Perez-Sanchez, E. Ensor, P. J. Morris, and D. S. Latchman Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression Nucleic Acids Res., December 2, 2006; 34(22): 6640 - 6652. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Hudson, P. J. Morris, D. S. Latchman, and V. S. Budhram-Mahadeo Brn-3a Transcription Factor Blocks p53-mediated Activation of Proapoptotic Target Genes Noxa and Bax in Vitro and in Vivo to Determine Cell Fate J. Biol. Chem., March 25, 2005; 280(12): 11851 - 11858. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Irshad, R. B. Pedley, J. Anderson, D. S. Latchman, and V. Budhram-Mahadeo The Brn-3b Transcription Factor Regulates the Growth, Behavior, and Invasiveness of Human Neuroblastoma Cells in Vitro and in Vivo J. Biol. Chem., May 14, 2004; 279(20): 21617 - 21627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Chao, J. N. Harada, F. Hyndman, X. Gao, C. G. Nelson, S. K. Chanda, and J. S. Caldwell PDX1, a Cellular Homeoprotein, Binds to and Regulates the Activity of Human Cytomegalovirus Immediate Early Promoter J. Biol. Chem., April 16, 2004; 279(16): 16111 - 16120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Weiss, I. Gottfried, I. Mayrose, S. L. Khare, M. Xiang, S. J. Dawson, and K. B. Avraham The DFNA15 Deafness Mutation Affects POU4F3 Protein Stability, Localization, and Transcriptional Activity Mol. Cell. Biol., November 15, 2003; 23(22): 7957 - 7964. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Jones Herpes Simplex Virus Type 1 and Bovine Herpesvirus 1 Latency Clin. Microbiol. Rev., January 1, 2003; 16(1): 79 - 95. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Perez-Sanchez, V. S. Budhram-Mahadeo, and D. S. Latchman Distinct promoter elements mediate the co-operative effect of Brn-3a and p53 on the p21 promoter and their antagonism on the Bax promoter Nucleic Acids Res., November 15, 2002; 30(22): 4872 - 4880. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Andersen and M. G. Rosenfeld POU Domain Factors in the Neuroendocrine System: Lessons from Developmental Biology Provide Insights into Human Disease Endocr. Rev., February 1, 2001; 22(1): 2 - 35. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Certel, P. Clyne, J. Carlson, and W. Johnson Regulation of central neuron synaptic targeting by the Drosophila POU protein, Acj6 Development, January 6, 2000; 127(11): 2395 - 2405. [Abstract] [PDF]
|
|
|
|
|
|
D. Ndisang, V. Budhram-Mahadeo, and D. S. Latchman The Brn-3a Transcription Factor Plays a Critical Role in Regulating Human Papilloma Virus Gene Expression and Determining the Growth Characteristics of Cervical Cancer Cells J. Biol. Chem., October 1, 1999; 274(40): 28521 - 28527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Budhram-Mahadeo, P. J. Morris, M. D. Smith, C. A. Midgley, L. M. Boxer, and D. S. Latchman p53 Suppresses the Activation of the Bcl-2 Promoter by the Brn-3a POU Family Transcription Factor J. Biol. Chem., May 21, 1999; 274(21): 15237 - 15244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Smith, E. A. Ensor, R. S. Coffin, L. M. Boxer, and D. S. Latchman Bcl-2 Transcription from the Proximal P2 Promoter Is Activated in Neuronal Cells by the Brn-3a POU Family Transcription Factor J. Biol. Chem., July 3, 1998; 273(27): 16715 - 16722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Budhram-Mahadeo, M. Parker, and D. S. Latchman POU Transcription Factors Brn-3a and Brn-3b Interact with the Estrogen Receptor and Differentially Regulate Transcriptional Activity via an Estrogen Response Element Mol. Cell. Biol., February 1, 1998; 18(2): 1029 - 1041. [Abstract] [Full Text]
|
|
|
|
|
|
M. D. Smith, P. J. Morris, S. J. Dawson, M. L. Schwartz, W. W. Schlaepfer, and D. S. Latchman Coordinate Induction of the Three Neurofilament Genes by the Brn-3a Transcription Factor J. Biol. Chem., August 22, 1997; 272(34): 21325 - 21333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A K Ryan and M G Rosenfeld POU domain family values: flexibility, partnerships, and developmental codes. Genes & Dev., May 15, 1997; 11(10): 1207 - 1225. [PDF]
|
|
|
|
|
|
M. D. Smith, S. J. Dawson, and D. S. Latchman Inhibition of Neuronal Process Outgrowth and Neuronal Specific Gene Activation by the Brn-3b Transcription Factor J. Biol. Chem., January 10, 1997; 272(2): 1382 - 1388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Pattyn, X Morin, H Cremer, C Goridis, and J. Brunet Expression and interactions of the two closely related homeobox genes Phox2a and Phox2b during neurogenesis Development, January 10, 1997; 124(20): 4065 - 4075. [Abstract] [PDF]
|
|
|
|
|
|
V. Budhram-Mahadeo, P. J. Morris, N. D. Lakin, S. J. Dawson, and D. S. Latchman The Different Activities of the Two Activation Domains of the Brn-3a Transcription Factor Are Dependent on the Context of the Binding Site J. Biol. Chem., April 12, 1996; 271(15): 9108 - 9113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. D. Lakin, P. J. Morris, T. Theil, T. N. Sato, T. Möröy, M. C. Wilson, and D. S. Latchman Regulation of Neurite Outgrowth and SNAP-25 Gene Expression by the Brn-3a Transcription Factor J. Biol. Chem., June 30, 1995; 270(26): 15858 - 15863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. G. N. Milton, A. Bessis, J.-P. Changeux, and D. S. Latchman The Neuronal Nicotinic Acetylcholine Receptor [IMAGE]2 Subunit Gene Promoter Is Activated by the Brn-3b POU Family Transcription Factor and Not by Brn-3a or Brn-3c J. Biol. Chem., June 23, 1995; 270(25): 15143 - 15147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Budhram-Mahadeo, P. J. Morris, N. D. Lakin, T. Theil, G. Y. Ching, K. A. Lillycrop, T. Möröy, R. K. H. Liem, and D. S. Latchman Activation of the alpha-Internexin Promoter by the Brn-3a Transcription Factor Is Dependent on the N-terminal Region of the Protein J. Biol. Chem., February 10, 1995; 270(6): 2853 - 2858. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Ensor, M. D. Smith, and D. S. Latchman The BRN-3A Transcription Factor Protects Sensory but Not Sympathetic Neurons from Programmed Cell Death/Apoptosis J. Biol. Chem., February 9, 2001; 276(7): 5204 - 5212. [Abstract] [Full Text] [PDF]
|
|