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Nucleic Acids Research, 1992, Vol. 20, No. 19 5131-5136
© 1992

CHEMISTRY

Synthesis of backbone deuterium labelled [r(CGCGAAUUCGCG)]2 and HPLC purification of synthetic RNA

Devesh Khare and John Orban*

Maryland Biotechnology Institute, University of Maryland, Center for Advanced Research in Biotechnology 9600 Gudelsky Drive, Rockville, MD 20850, USA

* To whom correspondence should be addressed

Received June 17, 1992. Accepted August 31, 1992.

The chemical synthesis of backbone deuterium labelled [r(CGCGAAU*U*CGCG)]2(U* = [5'-2H]U) is described. An efficient purification procedure was developed using a polymeric reverse phase (PRP) HPLC column at 60°C. This procedure provided pure RNA dodecamer in the multi-milligram quantities (39% overall yield) necessary for dynamics studies using solid-state deuterium NMR. The purification method has been effectively applied to other RNA sequences and will assist biophysical studies which require relatively large quantities of RNA oligomers.


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