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Nucleic Acids Research, 1992, Vol. 20, No. 2 171-178
© 1992

MOLECULAR BIOLOGY

Methylation of an {alpha}-foetoprotein gene intragenic site modulates gene activity

Karin Opdecamp, Michèle Rivière, Montsé Molné, Josiane Szpirer and Claude Szpirer*

Département de Biologie Moléculaire, Université Libre de Bruxelies, Rue des Chevaux 67, B-1640 Rhode-St-Genèse, Belgium

*To whom correspondence should be addressed

Received November 29, 1991. Accepted December 23, 1991.

By comparing the methylation pattern of Mspl/Hpall sites in the 5' region off the mouse {alpha}-foetoprotein (AFP) gene of different cells (hepatoma cells, foetal and adult liver, fibroblasts), we found a correlation between gene expression and unmethylation of a site located in the first intron of the gene. Other sites did not show this correlation, in tranfection experiments of unmethylated and methylated AFP-CAT chimeric constructions, we then showed that methylation of the intronic site negatively modulates expression of CAT activity. We also found that a DNA segment centered on this site binds nuclear proteins; however methylation did not affect protein binding.


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