Nucleic Acids Research, 1992, Vol. 20, No. 2 231-236
© 1992
MOLECULAR BIOLOGY |
The human insulin gene linked polymorphic region exhibits an altered DNA structure
1Department of Clinical Genetics, University of Birmingham, Birmingham Maternity Hospital Birmingham B15 2TG 2Department of Medicine, University of Birmingham, Queen Elizabeth Hospital Birmingham B15 2TH, UK 3Max-Planck-Institute for Molecular Genetics Ihnestrasse 63, D-1000 Berlin 33, FRG
Received October 29, 1991. Accepted December 16, 1991.
Regulation of transcription of the human insulin gene appears to involve a series of DNA sequences in the 5'region. Hypersensitivity to DNA structural probes has previously been demonstrated in regulatory regions of cloned genomic DNA fragments, and been correlated with gene activity. To investigate the structure of the DNA in the human insulin gene, bromoacetaldehyde and S1 nuclease were reacted with a supercoiled plasmid containing a 5kb genomic insulin fragment. Both probes revealed the human insulin gene linked polymorphic region (1LPR), a region (-363) upstream of the transcriptional start site which contains multiple repeats of a 14–15mer oligonucleotide with the consensus sequence ACAGGGGT(G/C)(T/C)GGGG, as the major hypersensitive site. Fine mapping and electron microscopic analysis both show a very different behaviour of the two DNA strands in the region of the ILPR and suggest the G-rich strand may be adopting a highly structured conformation with the complementary strand remaining largely single stranded.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. L. Palumbo, R. M. Memmott, D. J. Uribe, Y. Krotova-Khan, L. H. Hurley, and S. W. Ebbinghaus A novel G-quadruplex-forming GGA repeat region in the c-myb promoter is a critical regulator of promoter activity Nucleic Acids Res., April 1, 2008; 36(6): 1755 - 1769. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. W. Hay and K. Docherty Comparative Analysis of Insulin Gene Promoters: Implications for Diabetes Research Diabetes, December 1, 2006; 55(12): 3201 - 3213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Dai, D. Chen, R. A. Jones, L. H. Hurley, and D. Yang NMR solution structure of the major G-quadruplex structure formed in the human BCL2 promoter region Nucleic Acids Res., October 6, 2006; 34(18): 5133 - 5144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ambrus, D. Chen, J. Dai, T. Bialis, R. A. Jones, and D. Yang Human telomeric sequence forms a hybrid-type intramolecular G-quadruplex structure with mixed parallel/antiparallel strands in potassium solution. Nucleic Acids Res., January 1, 2006; 34(9): 2723 - 2735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Etzioni, A. Yafe, S. Khateb, P. Weisman-Shomer, E. Bengal, and M. Fry Homodimeric MyoD Preferentially Binds Tetraplex Structures of Regulatory Sequences of Muscle-specific Genes J. Biol. Chem., July 22, 2005; 280(29): 26805 - 26812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Yafe, S. Etzioni, P. Weisman-Shomer, and M. Fry Formation and properties of hairpin and tetraplex structures of guanine-rich regulatory sequences of muscle-specific genes Nucleic Acids Res., May 20, 2005; 33(9): 2887 - 2900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Vafiadis, H. Ounissi-Benkalha, M. Palumbo, R. Grabs, M. Rousseau, C. G. Goodyer, and C. Polychronakos Class III Alleles of the Variable Number of Tandem Repeat Insulin Polymorphism Associated with Silencing of Thymic Insulin Predispose to Type 1 Diabetes J. Clin. Endocrinol. Metab., August 1, 2001; 86(8): 3705 - 3710. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Lew, W. J. Rutter, and G. C. Kennedy Unusual DNA structure of the diabetes susceptibility locus IDDM2 and its effect on transcription by the insulin promoter factor Pur-1/MAZ PNAS, November 7, 2000; 97(23): 12508 - 12512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Simonsson and R. Sjoback DNA Tetraplex Formation Studied with Fluorescence Resonance Energy Transfer J. Biol. Chem., June 11, 1999; 274(24): 17379 - 17383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Harrington, Y. Lan, and S. A. Akman The Identification and Characterization of a G4-DNA Resolvase Activity J. Biol. Chem., September 26, 1997; 272(39): 24631 - 24636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. N. Weitzmann, K. J. Woodford, and K. Usdin DNA Secondary Structures and the Evolution of Hypervariable Tandem Arrays J. Biol. Chem., April 4, 1997; 272(14): 9517 - 9523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. N. Weitzmann, K. J. Woodford, and K. Usdin The Development and Use of a DNA Polymerase Arrest Assay for the Evaluation of Parameters Affecting Intrastrand Tetraplex Formation J. Biol. Chem., August 23, 1996; 271(34): 20958 - 20964. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Frantz and W. Gilbert A Yeast Gene Product, G4p2, with a Specific Affinity for Quadruplex Nucleic Acids J. Biol. Chem., April 21, 1995; 270(16): 9413 - 9419. [Abstract] [Full Text] [PDF]
|
|