Nucleic Acids Research

This Article Print PDF (6661K) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to My Personal Archive Download to citation manager Search for citing articles in: ISI Web of Science (83) Commercial Re-use Guidelines for Open Access NAR Content Google Scholar Articles by Tan, S.-H. Articles by Bernard, H.-U. Search for Related Content PubMed PubMed Citation Articles by Tan, S.-H. Articles by Bernard, H.-U. Social Bookmarking          What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 2 251-256
© 1992

MOLECULAR BIOLOGY

During negative regulation of the human papillomavirus-16E6 promoter, the viral E2 protein can displace Sp1 from a proximal promoter element

Shyh-Han Tan, Bernd Gloss and Hans-Ulrich Bernard

Institute of Molecular and Cell Biology, National University of Singapore Republic of Singapore 0511

Received September 17, 1991. Revised December 23, 1991. Accepted December 23, 1991.

The principal early promoter of human papillo-maviruses (HPVs), designated P97 in the case of HPV-16, contains four characteristically aligned cis-responsive elements, namely one binding site for Sp1, two for the viral E2 proteins, and the TATA box. The Sp1 binding site is needed to mediate activation of P97 by the remote epithelial-specific enhancer, and the two E2 binding sites contribute to a negative feedback-loop of viral gene expression. The Sp1 consensus motif and the TATA-box distal E2 binding site are spaced on all genital papillomaviruses by a single nucleotode. We show here that at physiological concentrations, the binding of E2 proteins and Sp1 are mutually exclusive events, since a bandshift analysis with nuclear extracts from ID13, a mouse cell line transformed by BPV-1, showed only the E2 or the Sp1 bandshift, but no complex indicative of the concomitant binding of both factors. Increasing concentrations of in vitro translated E2 protein compete efficiently with the Sp1 factor for binding to an oligonucleotide containing both binding sites. Interference between Sp1 and E2 protein binding is apparently relevant for P97 repression in vivo, since a mutational analysis revealed that both E2 binding sites are necessary for negative transcriptional regulation: Alone, neither the distal site, where E2 protein can induce Spi displacement, nor the proximal site, where E2 protein interferes with formation and function of the pre-initiation complex, have a significant effect, but two functional E2 binding sites lead to repression of P97.

CiteULike    Connotea    Del.icio.us    What's this?

This article has been cited by other articles:

				M. G. McPhillips, J. G. Oliveira, J. E. Spindler, R. Mitra, and A. A. McBride Brd4 Is Required for E2-Mediated Transcriptional Activation but Not Genome Partitioning of All Papillomaviruses J. Virol., October 1, 2006; 80(19): 9530 - 9543. [Abstract] [Full Text] [PDF]

				T. M. Wise-Draper, H. V. Allen, M. N. Thobe, E. E. Jones, K. B. Habash, K. Munger, and S. I. Wells The Human DEK Proto-Oncogene Is a Senescence Inhibitor and an Upregulated Target of High-Risk Human Papillomavirus E7 J. Virol., November 15, 2005; 79(22): 14309 - 14317. [Abstract] [Full Text] [PDF]

				S. Blachon, S. Bellanger, C. Demeret, and F. Thierry Nucleo-cytoplasmic Shuttling of High Risk Human Papillomavirus E2 Proteins Induces Apoptosis J. Biol. Chem., October 28, 2005; 280(43): 36088 - 36098. [Abstract] [Full Text] [PDF]

				W. G. Hubert Variant Upstream Regulatory Region Sequences Differentially Regulate Human Papillomavirus Type 16 DNA Replication throughout the Viral Life Cycle J. Virol., May 15, 2005; 79(10): 5914 - 5922. [Abstract] [Full Text] [PDF]

				K. M. Spink and L. A. Laimins Induction of the Human Papillomavirus Type 31 Late Promoter Requires Differentiation but Not DNA Amplification J. Virol., April 15, 2005; 79(8): 4918 - 4926. [Abstract] [Full Text] [PDF]

				E. Sen, S. Alam, and C. Meyers Genetic and Biochemical Analysis of cis Regulatory Elements within the Keratinocyte Enhancer Region of the Human Papillomavirus Type 31 Upstream Regulatory Region during Different Stages of the Viral Life Cycle J. Virol., January 15, 2004; 78(2): 612 - 629. [Abstract] [Full Text] [PDF]

				S. I. Wells, B. J. Aronow, T. M. Wise, S. S. Williams, J. A. Couget, and P. M. Howley Transcriptome signature of irreversible senescence in human papillomavirus-positive cervical cancer cells PNAS, June 10, 2003; 100(12): 7093 - 7098. [Abstract] [Full Text] [PDF]

				V. Bechtold, P. Beard, and K. Raj Human Papillomavirus Type 16 E2 Protein Has No Effect on Transcription from Episomal Viral DNA J. Virol., February 1, 2003; 77(3): 2021 - 2028. [Abstract] [Full Text] [PDF]

				A. Muller, A. Ritzkowsky, and G. Steger Cooperative Activation of Human Papillomavirus Type 8 Gene Expression by the E2 Protein and the Cellular Coactivator p300 J. Virol., October 2, 2002; 76(21): 11042 - 11053. [Abstract] [Full Text] [PDF]

				K. A. Hartley and K. A. Alexander Human TATA Binding Protein Inhibits Human Papillomavirus Type 11 DNA Replication by Antagonizing E1-E2 Protein Complex Formation on the Viral Origin of Replication J. Virol., April 16, 2002; 76(10): 5014 - 5023. [Abstract] [Full Text] [PDF]

				F. Stubenrauch, T. Zobel, and T. Iftner The E8 Domain Confers a Novel Long-Distance Transcriptional Repression Activity on the E8^E2C Protein of High-Risk Human Papillomavirus Type 31 J. Virol., May 1, 2001; 75(9): 4139 - 4149. [Abstract] [Full Text]

				K. W. Vance, M. S. Campo, and I. M. Morgan A Novel Silencer Element in the Bovine Papillomavirus Type 4 Promoter Represses the Transcriptional Response to Papillomavirus E2 Protein J. Virol., March 15, 2001; 75(6): 2829 - 2838. [Abstract] [Full Text]

				C. D. Newhouse and S. J. Silverstein Orientation of a Novel DNA Binding Site Affects Human Papillomavirus-Mediated Transcription and Replication J. Virol., February 15, 2001; 75(4): 1722 - 1735. [Abstract] [Full Text]

				A. Nishimura, T. Ono, A. Ishimoto, J. J. Dowhanick, M. A. Frizzell, P. M. Howley, and H. Sakai Mechanisms of Human Papillomavirus E2-Mediated Repression of Viral Oncogene Expression and Cervical Cancer Cell Growth Inhibition J. Virol., April 15, 2000; 74(8): 3752 - 3760. [Abstract] [Full Text]

				S. Chupreta, M. Du, A. Todisco, and J. L. Merchant EGF stimulates gastrin promoter through activation of Sp1 kinase activity Am J Physiol Cell Physiol, April 1, 2000; 278(4): C697 - C708. [Abstract] [Full Text] [PDF]

				P.-F. Su, S.-Y. Chiang, C.-W. Wu, and F. Y.-H. Wu Adeno-Associated Virus Major Rep78 Protein Disrupts Binding of TATA-Binding Protein to the p97 Promoter of Human Papillomavirus Type 16 J. Virol., March 1, 2000; 74(5): 2459 - 2465. [Abstract] [Full Text]

				N. Alloul and L. Sherman Transcription-modulatory activities of differentially spliced cDNAs encoding the E2 protein of human papillomavirus type 16 J. Gen. Virol., September 1, 1999; 80(9): 2461 - 2470. [Abstract] [Full Text]

				J. Karjalainen, U. M. Kujala, A. Stolt, M. Mantysaari, M. Viitasalo, K. Kainulainen, and K. Kontula Angiotensinogen gene M235T polymorphism predicts left ventricular hypertrophy in endurance athletes J. Am. Coll. Cardiol., August 1, 1999; 34(2): 494 - 499. [Abstract] [Full Text] [PDF]

				H. Lewis, K. Webster, A.-M. Sanchez-Perez, and K. Gaston Cellular transcription factors regulate human papillomavirus type 16 gene expression by binding to a subset of the DNA sequences recognized by the viral E2 protein J. Gen. Virol., August 1, 1999; 80(8): 2087 - 2096. [Abstract] [Full Text]

				M. J. O'Connor, W. Stunkel, H. Zimmermann, C.-H. Koh, and H.-U. Bernard A Novel YY1-Independent Silencer Represses the Activity of the Human Papillomavirus Type 16 Enhancer J. Virol., December 1, 1998; 72(12): 10083 - 10092. [Abstract] [Full Text] [PDF]

				F. Stubenrauch, A. M. E. Colbert, and L. A. Laimins Transactivation by the E2 Protein of Oncogenic Human Papillomavirus Type 31 Is Not Essential for Early and Late Viral Functions J. Virol., October 1, 1998; 72(10): 8115 - 8123. [Abstract] [Full Text] [PDF]

				E. C. Goodwin, L. K. Naeger, D. E. Breiding, E. J. Androphy, and D. DiMaio Transactivation-Competent Bovine Papillomavirus E2 Protein Is Specifically Required for Efficient Repression of Human Papillomavirus Oncogene Expression and for Acute Growth Inhibition of Cervical Carcinoma Cell Lines J. Virol., May 1, 1998; 72(5): 3925 - 3934. [Abstract] [Full Text] [PDF]

				I. Morgan, G. Grindlay, and M. Campo Epithelial specific transcriptional regulation of the bovine papillomavirus 4 promoter by E2 J. Gen. Virol., March 1, 1998; 79(3): 501 - 508. [Abstract]

				A. Thain, K. Webster, D. Emery, A. R. Clarke, and K. Gaston DNA Binding and Bending by the Human Papillomavirus Type 16E2 Protein. RECOGNITION OF AN EXTENDED BINDING SITE J. Biol. Chem., March 28, 1997; 272(13): 8236 - 8242. [Abstract] [Full Text] [PDF]

Online ISSN 1362-4962 - Print ISSN 0305-1048

Copyright © 2008 Oxford University Press

Oxford Journals Oxford University Press

• Site Map
• Privacy Policy
• Frequently Asked Questions

Other Oxford University Press sites: Oxford University Press Oxford Journals Japan American National Biography Booksellers' Information Service Children's Fiction and Poetry Children's Reference Corporate & Special Sales Dictionaries Dictionary of National Biography Digital Reference English Language Teaching Higher Education Textbooks Humanities International Education Unit Law Medicine Music Online Products Oxford English Dictionary Reference Rights and Permissions Science School Books Social Sciences Very Short Introductions World's Classics