Nucleic Acids Research, 1992, Vol. 20, No. 2 279-285
© 1992
MOLECULAR BIOLOGY |
In vitro definition of the yeast RNA polymerase I promoter
Hutchinson Cancer Research Center 1124 Columbia Street, Seattle, WA 98104, USA
*To whom correspondence should be addressed
Received September 24, 1991. Revised December 6, 1991. Accepted December 6, 1991.
The structure of the ribosomal gene promoter from Saccharomyces cerevisiae has been analyzed in a whole cell in vitro extract. The promoter contains at least two essential domains, an upstream domain located at the 5' boundary near position –150 and a core promoter domain around the site of transcription initiation at +1. The upstream domain augments transcription in vitro but is not absolutely required. Maintenance of correct spacing between the two domains is critical. The in vitro analysis agrees well with prior in vivo analysis and it appears that the yeast promoter has a structure very similar to that of vertebrate ribosomal gene promoters.
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