Nucleic Acids Research, 1992, Vol. 20, No. 2 295-301
© 1992
MOLECULAR BIOLOGY |
A new rRNA processing mutant of Saccharomyces cerevisiae
Department of Biology, University of Rochester Rochester, NY 14627, USA
Received September 16, 1991. Revised December 16, 1991. Accepted December 16, 1991.
We have identified from a collection of temperature sensitive yeast mytants strains which fail to process rRNA normally. Characterization of one such mutant is reported here. This strain accumulates increased amounts of the 35S primary transcript, ‘24S’ molecules extending from the transcription start site to the 5.8S region, and two classes of 5.8S rRNA with 5' extensions of 7 and 149 bases, respectively. We show that this pleiotropic change in the rRNA processing pattern is dye to a single mutation. Possoble models for the function of the mutated gene are discussed.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. S. Bommakanti, L. Lindahl, and J. M. Zengel Mutation from guanine to adenine in 25S rRNA at the position equivalent to E. coli A2058 does not confer erythromycin sensitivity in Sacchromyces cerevisae RNA, March 1, 2008; 14(3): 460 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Xiao, J. Hsieh, R. L. Nugent, D. J. Coughlin, C. A. Fierke, and D. R. Engelke Functional characterization of the conserved amino acids in Pop1p, the largest common protein subunit of yeast RNases P and MRP RNA, June 1, 2006; 12(6): 1023 - 1037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. FABER, J. C. VOS, H. R. VOS, G. GHAZAL, S. ABOU ELELA, and H. A. RAUE The RNA catabolic enzymes Rex4p, Rnt1p, and Dbr1p show genetic interaction with trans-acting factors involved in processing of ITS1 in Saccharomyces cerevisiae pre-rRNA RNA, December 1, 2004; 10(12): 1946 - 1956. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Li, S. Zaman, Y. Langdon, J. M. Zengel, and L. Lindahl Identification of a functional core in the RNA component of RNase MRP of budding yeasts Nucleic Acids Res., July 14, 2004; 32(12): 3703 - 3711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. van Beekvelt, M. de Graaff-Vincent, A. W. Faber, J. van 't Riet, J. Venema, and H. A. Raue All three functional domains of the large ribosomal subunit protein L25 are required for both early and late pre-rRNA processing steps in Saccharomyces cerevisiae Nucleic Acids Res., December 15, 2001; 29(24): 5001 - 5008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Allmang, P. Mitchell, E. Petfalski, and D. Tollervey Degradation of ribosomal RNA precursors by the exosome Nucleic Acids Res., April 15, 2000; 28(8): 1684 - 1691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Kressler, P. Linder, and J. de la Cruz Protein trans-Acting Factors Involved in Ribosome Biogenesis in Saccharomyces cerevisiae Mol. Cell. Biol., December 1, 1999; 19(12): 7897 - 7912. [Full Text] [PDF]
|
|
|
|
|
|
Y. Zebarjadian, T. King, M. J. Fournier, L. Clarke, and J. Carbon Point Mutations in Yeast CBF5 Can Abolish In Vivo Pseudouridylation of rRNA Mol. Cell. Biol., November 1, 1999; 19(11): 7461 - 7472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. Briggs, K. T. D. Burkard, and J. S. Butler Rrp6p, the Yeast Homologue of the Human PM-Scl 100-kDa Autoantigen, Is Essential for Efficient 5.8 S rRNA 3' End Formation J. Biol. Chem., May 22, 1998; 273(21): 13255 - 13263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Stolc and S. Altman Rpp1, an essential protein subunit of nuclear RNase P required for processing of precursor tRNA and 35S precursor rRNA in Saccharomyces cerevisiae Genes & Dev., November 1, 1997; 11(21): 2926 - 2937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Stolc and S. Altman Rpp1, an essential protein subunit of nuclear RNase P required for processing of precursor tRNA and 35S precursor rRNA in Saccharomyces cerevisiae Genes & Dev., September 15, 1997; 11(18): 2414 - 2425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P Mitchell, E Petfalski, and D Tollervey The 3' end of yeast 5.8S rRNA is generated by an exonuclease processing mechanism. Genes & Dev., February 15, 1996; 10(4): 502 - 513. [Abstract] [PDF]
|
|
|
|
|
|
M E Schmitt and D A Clayton Characterization of a unique protein component of yeast RNase MRP: an RNA-binding protein with a zinc-cluster domain. Genes & Dev., November 1, 1994; 8(21): 2617 - 2628. [Abstract] [PDF]
|
|
|
|
|
|
Z Lygerou, P Mitchell, E Petfalski, B Seraphin, and D Tollervey The POP1 gene encodes a protein component common to the RNase MRP and RNase P ribonucleoproteins. Genes & Dev., June 15, 1994; 8(12): 1423 - 1433. [Abstract] [PDF]
|
|