Nucleic Acids Research, 1992, Vol. 20, No. 20 5271-5278
© 1992
MOLECULAR BIOLOGY |
Yeast SKO1 gene encodes a bZIP protein that binds to the CRE motif and acts as a repressor of transcription
Ludwig Institute for Cancer Research, Uppsala Branch Biomedical Center, Box 595, S-751 24 Uppsala, Sweden
* To whom correspondence should be addressed
Received August 17, 1992. Accepted September 22, 1992.
We have cloned a yeast gene, SKO1, which in high copy number suppresses lethal overexpresslon of cAMP-dependent protein kinase. SK01 encodes a bZIP protein that binds to the CRE motif, TGACGTCA. We found that SKO1 also binds to a CRE-like site in SUC2, a yeast gene encoding invertase which Is under positive control by cAMP. A disruption of the SKO1 gene causes a partial derepression of SUC2, Indicating that SKO1 Is a negative regulator of the SUC2 gene. SKO1 interacts positively with MIG1, a zinc finger protein that mediates glucose repression of SUC2. A kinetic analysis revealed a complex regulation of the SUC2 mRNA in response to glucose. First, MIG1 mediates a rapid and strong repression of SUC2, which is complete within 10 minutes. Second, a MIG1-independent process causes a further slow reduction In the mRNA. Third, in the absence of MIG1, there is also a rapid but transient glucose induction of the SUC2 mRNA. This induction is correlated with a transient loss of SKO1-dependent repression.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Gao, M. K. Davidson, and W. P. Wahls Distinct regions of ATF/CREB proteins Atf1 and Pcr1 control recombination hotspot ade6-M26 and the osmotic stress response Nucleic Acids Res., May 1, 2008; 36(9): 2838 - 2851. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Guha, P. Desai, and A. Vancura Plc1p Is Required for SAGA Recruitment and Derepression of Sko1p-regulated Genes Mol. Biol. Cell, July 1, 2007; 18(7): 2419 - 2428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zapater, M. Sohrmann, M. Peter, F. Posas, and E. de Nadal Selective Requirement for SAGA in Hog1-Mediated Gene Expression Depending on the Severity of the External Osmostress Conditions Mol. Cell. Biol., June 1, 2007; 27(11): 3900 - 3910. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Deminoff, S. C. Howard, A. Hester, S. Warner, and P. K. Herman Using Substrate-Binding Variants of the cAMP-Dependent Protein Kinase to Identify Novel Targets and a Kinase Domain Important for Substrate Interactions in Saccharomyces cerevisiae Genetics, August 1, 2006; 173(4): 1909 - 1917. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Boukaba, E. I. Georgieva, F. A. Myers, A. W. Thorne, G. Lopez-Rodas, C. Crane-Robinson, and L. Franco A Short-range Gradient of Histone H3 Acetylation and Tup1p Redistribution at the Promoter of the Saccharomyces cerevisiae SUC2 Gene J. Biol. Chem., February 27, 2004; 279(9): 7678 - 7684. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hohmann Osmotic Stress Signaling and Osmoadaptation in Yeasts Microbiol. Mol. Biol. Rev., June 1, 2002; 66(2): 300 - 372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pascual-Ahuir, R. Serrano, and M. Proft The Sko1p Repressor and Gcn4p Activator Antagonistically Modulate Stress-Regulated Transcription in Saccharomyces cerevisiae Mol. Cell. Biol., January 1, 2001; 21(1): 16 - 25. [Abstract] [Full Text]
|
|
|
|
|
|
M. A. Garcia-Gimeno and K. Struhl Aca1 and Aca2, ATF/CREB Activators in Saccharomyces cerevisiae, Are Important for Carbon Source Utilization but Not the Response to Stress Mol. Cell. Biol., June 15, 2000; 20(12): 4340 - 4349. [Abstract] [Full Text]
|
|
|
|
|
|
E. Bilsland-Marchesan, J. Ariño, H. Saito, P. Sunnerhagen, and F. Posas Rck2 Kinase Is a Substrate for the Osmotic Stress-Activated Mitogen-Activated Protein Kinase Hog1 Mol. Cell. Biol., June 1, 2000; 20(11): 3887 - 3895. [Abstract] [Full Text]
|
|
|
|
|
|
M. Proft and R. Serrano Repressors and Upstream Repressing Sequences of the Stress-Regulated ENA1 Gene in Saccharomyces cerevisiae: bZIP Protein Sko1p Confers HOG-Dependent Osmotic Regulation Mol. Cell. Biol., January 1, 1999; 19(1): 537 - 546. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Gancedo Yeast Carbon Catabolite Repression Microbiol. Mol. Biol. Rev., June 1, 1998; 62(2): 334 - 361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Liang and T. Hai Characterization of Human Activating Transcription Factor 4, a Transcriptional Activator That Interacts with Multiple Domains of cAMP-responsive Element-binding Protein (CREB)-binding Protein (CBP) J. Biol. Chem., September 19, 1997; 272(38): 24088 - 24095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. T. Coleman, E. Tseng, and W. S. Moye-Rowley Saccharomyces cerevisiae Basic Region-Leucine Zipper Protein Regulatory Networks Converge at the ATR1 Structural Gene J. Biol. Chem., September 12, 1997; 272(37): 23224 - 23230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pascual-Ahuir, F. Posas, R. Serrano, and M. Proft Multiple Levels of Control Regulate the Yeast cAMP-response Element-binding Protein Repressor Sko1p in Response to Stress J. Biol. Chem., September 28, 2001; 276(40): 37373 - 37378. [Abstract] [Full Text] [PDF]
|
|