Skip Navigation

This Article
Right arrow Print PDF (2208K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (46)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Isomura, T.
Right arrow Articles by Takahashi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Isomura, T.
Right arrow Articles by Takahashi, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 20 5305-5310
© 1992

MOLECULAR BIOLOGY

RFP is a DNA binding protein associated with the nuclear matrix

Takeshi Isomura1,2, Keiko Tamiya-Koizumi3, Motoshi Suzuki3, Shonen Yoshida3, Masahiko Taniguchi1, Mutsushi Matsuyama1, Takeo Ishigaki2, Sadayuki Sakuma2 and Masahide Takahashi1,*

1Department of Pathology, Nagoya University School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan 2Department of Radiology, Nagoya University School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan 3Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan

* To whom correspondence should be addressed

Received July 24, 1992. Revised September 25, 1992. Accepted September 25, 1992.

We reported that the RFP gene encodes a protein with putative zinc finger domains and was involved In the activation of the ret proto-oncogene. To further characterize the RFP protein, we developed a polyclonal antibody against the product synthesized from a fragment of the RFP cDNA expressed in Escherichia coll. Western blot analysis showed that RFP was identified as a 58 kDa protein In cell lysates from four human and rodent cell lines and from mouse test is. In addition, a unique 68 kDa protein was detected in the testis. Using AH7974 (rat ascites hepatoma) and Raji (human Burkttt lymphoma) cells, we demonstrated strong association of RFP with the nuclear matrix. Furthermore, RFP solubilized from the nuclear matrix had DNA-bindlng activity although It appears to bind more preferentially to double-stranded DNA than to single-stranded DNA. These results thus suggest that RFP may play a role in molecular processes which occur in the nuclear matrix.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. Harbers, T. Nomura, S. Ohno, and S. Ishii
Intracellular Localization of the Ret Finger Protein Depends on a Functional Nuclear Export Signal and Protein Kinase C Activation
J. Biol. Chem., December 14, 2001; 276(51): 48596 - 48607.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. H. Charpentier, A. K. Bednarek, R. L. Daniel, K. A. Hawkins, K. J. Laflin, S. Gaddis, M. C. MacLeod, and C. M. Aldaz
Effects of Estrogen on Global Gene Expression: Identification of Novel Targets of Estrogen Action
Cancer Res., November 1, 2000; 60(21): 5977 - 5983.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
H. Harada, Y. Harada, D. P. O'Brien, D. S. Rice, C. W. Naeve, and J. R. Downing
HERF1, a Novel Hematopoiesis-Specific RING Finger Protein, Is Required for Terminal Differentiation of Erythroid Cells
Mol. Cell. Biol., May 1, 1999; 19(5): 3808 - 3815.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Schweiger, J. Foerster, T. Lehmann, V. Suckow, Y. A. Muller, G. Walter, T. Davies, H. Porter, H. van Bokhoven, P. W. Lunt, et al.
The Opitz syndrome gene product, MID1, associates with microtubules
PNAS, March 16, 1999; 96(6): 2794 - 2799.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
D. A. Ruddy, G. S. Kronmal, V. K. Lee, G. A. Mintier, L. Quintana, R. Domingo Jr., N. C. Meyer, A. Irrinki, E. E. McClelland, A. Fullan, et al.
A 1.1-Mb Transcript Map of the Hereditary Hemochromatosis Locus
Genome Res., May 1, 1997; 7(5): 441 - 456.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. E. Jackson, D. O' Neill, and A. Bank
Nuclear Factor Binding Sites in Human beta Globin IVS2
J. Biol. Chem., November 24, 1995; 270(47): 28448 - 28456.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Shimono, H. Murakami, Y. Hasegawa, and M. Takahashi
RET Finger Protein Is a Transcriptional Repressor and Interacts with Enhancer of Polycomb That Has Dual Transcriptional Functions
J. Biol. Chem., December 8, 2000; 275(50): 39411 - 39419.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.