Nucleic Acids Research, 1992, Vol. 20, No. 20 5423-5428
© 1992
MOLECULAR BIOLOGY |
Elements of an archaeal promoter defined by mutational analysis
Max-Planck-Institut fü Biochemie 8033 Martinsried, Germany
* To whom correspondence should be addressed
Received June 24, 1992. Revised September 25, 1992. Accepted September 25, 1992.
The sequence requirements for specific and efficient transcription from the 16S/23S rRNA promoter of Sulfolobus shibatae were analysed by point mutations and by cassette mutations using an in vitro transcription system. The examination of the box A-containing distal promoter element (DPE) showed the great importance of the TA sequence in the center of box A for transcription efficiency and the influence of the sequence upstream of box A on determining the distance between the DPE and the start site. In most positions of box A, replacement of the wild type bases by adenines or thymlnes are less detrimental than replacements by cytosines or guanines. The effectiveness of the proximal promoter element (PPE) was not merely determined by its high A + T content but appeared to be directly related to its nucleotide sequence. At the start site a pyrimldlne/purine (py/pu) sequence was necessary for unambiguous initiation as shown by analysis of mutants where the wild type start base was replaced. The sequence of box A optimal for promoter function in vitro is identical to the consensus of 84 mapped archaeal promoter sequences.
+ Present address: MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312, USA
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  U. Kappler, L. I. Sly, and A. G. McEwan Respiratory gene clusters of Metallosphaera sedula - differential expression and transcriptional organization Microbiology, January 1, 2005; 151(1): 35 - 43. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Bartlett, M. Thomm, and E. P. Geiduschek Topography of the Euryarchaeal Transcription Initiation Complex J. Biol. Chem., February 13, 2004; 279(7): 5894 - 5903. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Schelert, V. Dixit, V. Hoang, J. Simbahan, M. Drozda, and P. Blum Occurrence and Characterization of Mercury Resistance in the Hyperthermophilic Archaeon Sulfolobus solfataricus by Use of Gene Disruption J. Bacteriol., January 15, 2004; 186(2): 427 - 437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-L. Thia-Toong, M. Roovers, V. Durbecq, D. Gigot, N. Glansdorff, and D. Charlier Genes of De Novo Pyrimidine Biosynthesis from the Hyperthermoacidophilic Crenarchaeote Sulfolobus acidocaldarius: Novel Organization in a Bipolar Operon J. Bacteriol., August 15, 2002; 184(16): 4430 - 4441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kähler and G. Antranikian Cloning and Characterization of a Family B DNA Polymerase from the Hyperthermophilic Crenarchaeon Pyrobaculum islandicum J. Bacteriol., February 1, 2000; 182(3): 655 - 663. [Abstract] [Full Text]
|
|
|
|
|
|
N. J. Cosper, C. M. V. Stalhandske, H. Iwasaki, T. Oshima, R. A. Scott, and T. Iwasaki Structural Conservation of the Isolated Zinc Site in Archaeal Zinc-containing Ferredoxins as Revealed by X-ray Absorption Spectroscopic Analysis and Its Evolutionary Implications J. Biol. Chem., August 13, 1999; 274(33): 23160 - 23168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kletzin, A. Lieke, T. Urich, R. L. Charlebois, and C. W. Sensen Molecular Analysis of pDL10 From Acidianus ambivalens Reveals a Family of Related Plasmids From Extremely Thermophilic and Acidophilic Archaea Genetics, August 1, 1999; 152(4): 1307 - 1314. [Abstract] [Full Text]
|
|
|
|
|
|
C. Haseltine, R. Montalvo-Rodriguez, A. Carl, E. Bini, and P. Blum Extragenic Pleiotropic Mutations That Repress Glycosyl Hydrolase Expression in the Hyperthermophilic Archaeon Sulfolobus solfataricus Genetics, August 1, 1999; 152(4): 1353 - 1361. [Abstract] [Full Text]
|
|
|
|
|
|
K. M. Stedman, C. Schleper, E. Rumpf, and W. Zillig Genetic Requirements for the Function of the Archaeal Virus SSV1 in Sulfolobus solfataricus: Construction and Testing of Viral Shuttle Vectors Genetics, August 1, 1999; 152(4): 1397 - 1405. [Abstract] [Full Text]
|
|
|
|
|
|
C. Haseltine, R. Montalvo-Rodriguez, E. Bini, A. Carl, and P. Blum Coordinate Transcriptional Control in the Hyperthermophilic Archaeon Sulfolobus solfataricus J. Bacteriol., July 1, 1999; 181(13): 3920 - 3927. [Abstract] [Full Text]
|
|
|
|
|
|
N. S. Baliga and S. DasSarma Saturation Mutagenesis of the TATA Box and Upstream Activator Sequence in the Haloarchaeal bop Gene Promoter J. Bacteriol., April 15, 1999; 181(8): 2513 - 2518. [Abstract] [Full Text]
|
|
|
|
|
|
C. Schleper, E. F. DeLong, C. M. Preston, R. A. Feldman, K.-Y. Wu, and R. V. Swanson Genomic Analysis Reveals Chromosomal Variation in Natural Populations of the Uncultured Psychrophilic Archaeon Cenarchaeum symbiosum J. Bacteriol., October 1, 1998; 180(19): 5003 - 5009. [Abstract] [Full Text]
|
|
|
|
|
|
N. Nomura, Y. Sako, and A. Uchida Molecular Characterization and Postsplicing Fate of Three Introns within the Single rRNA Operon of the Hyperthermophilic Archaeon Aeropyrum pernix K1 J. Bacteriol., July 15, 1998; 180(14): 3635 - 3643. [Abstract] [Full Text]
|
|
|
|
|
|
D. Prangishvili, H.-P. Klenk, G. Jakobs, A. Schmiechen, C. Hanselmann, I. Holz, and W. Zillig Biochemical and Phylogenetic Characterization of the dUTPase from the Archaeal Virus SIRV J. Biol. Chem., March 13, 1998; 273(11): 6024 - 6029. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Horlacher, K. B. Xavier, H. Santos, J. DiRuggiero, M. Kossmann, and W. Boos Archaeal Binding Protein-Dependent ABC Transporter: Molecular and Biochemical Analysis of the Trehalose/Maltose Transport System of the Hyperthermophilic Archaeon Thermococcus litoralis J. Bacteriol., February 1, 1998; 180(3): 680 - 689. [Abstract] [Full Text]
|
|
|
|
 |
|
 V. Durbecq, C. Legrain, M. Roovers, A. Pierard, and N. Glansdorff The carbamate kinase-like carbamoyl phosphate synthetase of the hyperthermophilic archaeon Pyrococcus furiosus, a missing link in the evolution of carbamoyl phosphate biosynthesis PNAS, November 25, 1997; 94(24): 12803 - 12808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. F. Kosa, G. Ghosh, B. S. DeDecker, and P. B. Sigler The 2.1-A crystal structure of an archaeal preinitiation complex: TATA-box-binding protein/transcription factor (II)B core/TATA-box PNAS, June 10, 1997; 94(12): 6042 - 6047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tauer and S. A. Benner The B12-dependent ribonucleotide reductase from the archaebacterium Thermoplasma acidophila: An evolutionary solution to the ribonucleotide reductase conundrum PNAS, January 7, 1997; 94(1): 53 - 58. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. LeClerc and D. A. Grahame Methylcobamide:Coenzyme M Methyltransferase Isozymes from Methanosarcina barkeri. PHYSICOCHEMICAL CHARACTERIZATION, CLONING, SEQUENCE ANALYSIS, AND HETEROLOGOUS GENE EXPRESSION J. Biol. Chem., August 2, 1996; 271(31): 18725 - 18731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Guagliardi, D. de Pascale, R. Cannio, V. Nobile, S. Bartolucci, and M. Rossi The Purification, Cloning, and High Level Expression of a Glutaredoxin-like Protein from the Hyperthermophilic Archaeon Pyrococcus furiosus J. Biol. Chem., March 17, 1995; 270(11): 5748 - 5755. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T Rowlands, P Baumann, and S. Jackson The TATA-binding protein: a general transcription factor in eukaryotes and archaebacteria Science, May 27, 1994; 264(5163): 1326 - 1329. [Abstract] [PDF]
|
|