Nucleic Acids Research, 1992, Vol. 20, No. 21 5593-5600
© 1992
MOLECULAR BIOLOGY |
Binding of Xenopus oocyte masking proteins to mRNA sequences
School of Biological and Medical Sciences, University of St Andrews St Andrews, Fife KY16 9TS, UK
* To whom correspondence should be addressed
Received August 26, 1992. Revised September 29, 1992. Accepted September 29, 1992.
It has been shown previously that maternal mRNA, synthesized and stored in growing oocytes, is stabilized and blocked from translation through various mechanisms including restricted polyadenylation and the binding of proteins to 3' regulatory elements. In addition to binding sequence-specific proteins, the bulk of stored mRNA is packaged with a set of ‘masking’ proteins, the most abundant of which are the phosphoproteins pp56 and pp60. In this report these proteins are shown to be bound to heterogeneous mRNA sequences and not to the 3' poly(A) tract. Crosslinking studies demonstrate that all of the pp56/60 present makes direct contact with the RNA. In vitro binding studies confirm that pp56/60 interact with single-stranded RNA of heterogeneous sequence, such as occurring in the maternal mRNA encoding cyclin B1. However, binding is equally effective to capped and polyadenylated cyclin mRNA, to truncated mRNA lacking 5' and 3' non-coding regions and even to the antisense sequence. Lengths of 70 – 80 nucleotides are protected from ribonuclease digestion after protein binding. Although no extended binding motif could be detected, binding does appear to have some specificity in that it is not competed out by 100-fold excess of double-stranded RNA, transfer RNA, poly(A) and various other homopolymers and heteropolymers. The sequence which competes most efficiently is the mixed polypyrimidine, poly(C,U). Crosslinking of RNA-protein complexes, followed by ribonuclease digestion, suggests that the arrangement of proteins on RNA is as dimers. Dimerization appears to be stabilized by phosphorylation of pp56/60. These results are discussed in terms of the known structures of pp56/60.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. A. Lewis, J. A. Gagnon, and K. L. Mowry PTB/hnRNP I Is Required for RNP Remodeling during RNA Localization in Xenopus Oocytes Mol. Cell. Biol., January 15, 2008; 28(2): 678 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. R. Belak and N. Ovsenek Assembly of the Yin Yang 1 Transcription Factor into Messenger Ribonucleoprotein Particles Requires Direct RNA Binding Activity J. Biol. Chem., December 28, 2007; 282(52): 37913 - 37920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G Manandhar, D Feng, Y-J Yi, L Lai, J Letko, J Laurincik, M Sutovsky, J L Salisbury, R S Prather, H Schatten, et al. Centrosomal protein centrin is not detectable during early pre-implantation development but reappears during late blastocyst stage in porcine embryos. Reproduction, September 1, 2006; 132(3): 423 - 434. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Weston and J. Sommerville Xp54 and related (DDX6-like) RNA helicases: roles in messenger RNP assembly, translation regulation and RNA degradation Nucleic Acids Res., June 14, 2006; 34(10): 3082 - 3094. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Claussen, K. Horvay, and T. Pieler Evidence for overlapping, but not identical, protein machineries operating in vegetal RNA localization along early and late pathways in Xenopus oocytes Development, September 1, 2004; 131(17): 4263 - 4273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Takiya, Y. Nishita, S. Ishikawa, K. Ohno, T.-a. Tamura, and Y. Suzuki Bombyx Y-Box Protein BYB Facilitates Specific DNA Interaction of Various DNA Binding Proteins Independently of the Cold Shock Domain J. Biochem., June 1, 2004; 135(6): 683 - 693. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-H. Tsai-Morris, Y. Sheng, E. Lee, K.-J. Lei, and M. L. Dufau Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is essential for spermatid development and completion of spermatogenesis PNAS, April 27, 2004; 101(17): 6373 - 6378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sanchez and W. F. Marzluff The Stem-Loop Binding Protein Is Required for Efficient Translation of Histone mRNA In Vivo and In Vitro Mol. Cell. Biol., October 15, 2002; 22(20): 7093 - 7104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. Pisarev, M. A. Skabkin, A. A. Thomas, W. C. Merrick, L. P. Ovchinnikov, and I. N. Shatsky Positive and Negative Effects of the Major Mammalian Messenger Ribonucleoprotein p50 on Binding of 40 S Ribosomal Subunits to the Initiation Codon of beta -Globin mRNA J. Biol. Chem., May 3, 2002; 277(18): 15445 - 15451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ficzycz and N. Ovsenek The Yin Yang 1 Transcription Factor Associates with Ribonucleoprotein (mRNP) Complexes in the Cytoplasm of Xenopus Oocytes J. Biol. Chem., March 1, 2002; 277(10): 8382 - 8387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Smillie and J. Sommerville RNA helicase p54 (DDX6) is a shuttling protein involved in nuclear assembly of stored mRNP particles J. Cell Sci., January 15, 2002; 115(2): 395 - 407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. Schmidt, E. S. Hanson, and M. R. Capecchi Sequence-Independent Assembly of Spermatid mRNAs into Messenger Ribonucleoprotein Particles Mol. Cell. Biol., May 1, 1999; 19(5): 3904 - 3915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Swamynathan, A. Nambiar, and R. V. Guntaka Role of single-stranded DNA regions and Y-box proteins in transcriptional regulation of viral and cellular genes FASEB J, May 1, 1998; 12(7): 515 - 522. [Abstract] [Full Text]
|
|
|
|
|
|
V. M. Evdokimova, E. A. Kovrigina, D. V. Nashchekin, E. K. Davydova, J. W. B. Hershey, and L. P. Ovchinnikov The Major Core Protein of Messenger Ribonucleoprotein Particles (p50) Promotes Initiation of Protein Biosynthesis in Vitro J. Biol. Chem., February 6, 1998; 273(6): 3574 - 3581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Matsumoto, F. Meric, and A. P. Wolffe Translational Repression Dependent on the Interaction of the Xenopus Y-box Protein FRGY2 with mRNA. ROLE OF THE COLD SHOCK DOMAIN, TAIL DOMAIN, AND SELECTIVE RNA SEQUENCE RECOGNITION J. Biol. Chem., September 13, 1996; 271(37): 22706 - 22712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Bouvet, K. Matsumoto, and A. P. Wolffe Sequence-specific RNA Recognition by the Xenopus Y-box Proteins J. Biol. Chem., November 24, 1995; 270(47): 28297 - 28303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. M. Evdokimova, C.-L. Wei, A. S. Sitikov, P. N. Simonenko, O. A. Lazarev, K. S. Vasilenko, V. A. Ustinov, J. W. B. Hershey, and L. P. Ovchinnikov The Major Protein of Messenger Ribonucleoprotein Particles in Somatic Cells Is a Member of the Y-box Binding Transcription Factor Family J. Biol. Chem., February 17, 1995; 270(7): 3186 - 3192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Robbie, M Peterson, E Amaya, and T. Musci Temporal regulation of the Xenopus FGF receptor in development: a translation inhibitory element in the 3' untranslated region Development, January 6, 1995; 121(6): 1775 - 1785. [Abstract] [PDF]
|
|
|
|
|
|
O. I. Stenina, K. M. Shaneyfelt, and P. E. DiCorleto Thrombin induces the release of the Y-box protein dbpB from mRNA: A mechanism of transcriptional activation PNAS, June 19, 2001; 98(13): 7277 - 7282. [Abstract] [Full Text] [PDF]
|
|