Nucleic Acids Research, 1992, Vol. 20, No. 21 5617-5623
© 1992
MOLECULAR BIOLOGY |
SPO12 and SIT4 suppress mutations in DBF2, which encodes a cell cycle protein kinase that is periodically expressed
Laboratory of Yeast Genetics, National Institute for Medical Research Mill Hill, London NW7 1AA, UK
* To whom correspondence should be addressed
Received August 21, 1992. Revised October 1, 1992. Accepted October 1, 1992.
To help clarify the role of DBF2, a previously described cell cycle protein kinase, high copy number suppressors of the dbf2 mutation were isolated. Three open reading frames (ORF) have been identified. One ORF encodes a protein which has homology to a human small nuclear riboprotein, while the remaining two are genes which have been identified previously, SIT4 and SPO12. SIT4 is known to have a role in the cell cycle but the nature of the interaction between SIT4 and dbf2 is unclear. SPO12 has until now been implicated exclusively in meiosis. However, we show that SPO12 is expressed during vegetative growth, moreover it is expressed under cell cycle control coordinately with DBF2. SPO12 is a nonessential gene, but it becomes essential in a DBF2 delete genetic background. Furthermore, detailed analysis of the cell cycle of SPO12 delete cells revealed a small but significant delay in mitosis. Therefore, SPO12 does have a role during vegetative growth and it probably functions in mitosis in association with DBF2.
+ Present address: Molecular Cell Pathology, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, UK
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