Skip Navigation

This Article
Right arrow Print PDF (458K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Artero, R. D.
Right arrow Articles by Pérez-Alonso, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Artero, R. D.
Right arrow Articles by Pérez-Alonso, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 21 5687-5690
© 1992

METHODS

Oligonucleotide probes detect splicing variants in situ in Drosophila embryos

Rubén D. Artero, Michael Akaml and Manuel Pérez-Alonso

Department of Genetics, University of Valencia, Campus of Burjasot E-46100 Burjasot, Spain 1Wellcome/CRC Institute of Cancer and Developmental Biology Tennis Court Road, Cambridge CB2 1QR, UK

Received July 23, 1992. Revised September 25, 1992. Accepted September 25, 1992.

We describe a method for the in situ detection of specific splicing variants. The method is based on the use of antisense oligonucleotides designed to span splice junctions labelled with digoxigenin by terminal transferase tailing. We find that the spatial patterns of Ubx splicing variants la and lla are similar in early embryos, but differ in late embryos. Variant IVa is only detected in the CNS (ps6) at stages 16 and 17. We also present evidence indicating that the first splicing event is cotranscriptional.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 DevelopmentHome page
J Castelli-Gair and M Akam
How the Hox gene Ultrabithorax specifies two different segments: the significance of spatial and temporal regulation within metameres
Development, January 9, 1995; 121(9): 2973 - 2982.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.