Nucleic Acids Research, 1992, Vol. 20, No. 21 5805-5810
© 1992
MOLECULAR BIOLOGY |
An ultraviolet light-damaged DNA recognition protein absent in xeroderma pigmentosum group E cells binds selectively to pyrimidine (6–4) pyrimidone photoproducts
Division of Toxicology and Department of Chemistry, Whitaker College of Health Sciences and Technology, Massachusetts Institute of Technology Cambridge, MA 02139, USA
* To whom correspondence should be addressed
Received June 17, 1992. Revised October 7, 1992. Accepted October 7, 1992.
The binding specificity was defined of a human ultraviolet light-damaged DNA recognition protein (UV-DRP), the activity of which is absent in some xeroderma pigmentosum complementation group E cells. Our results suggest that cyclobutane pyrimidine dimers (CPDs) are not high affinity UV-DRP binding sites—a finding consistent with other reports on this protein (Hirschfeld et al)., (1990) Mol. Cell Biol., 10, 2041 – 2048). A major role for 6–4 photoproducts in UV-DRP binding was suggested in studies showing that irradiated oligonucleotides containing a T4C UV box sequence, which efficiently forms a TC 6–4 photoproduct, was a superior substrate for the UV-DRP when compared to a similar irradiated oligonucleotide having a T5 sequence. The latter sequence forms CPDs at a much higher frequency than 6–4 photoproducts. In a more direct approach, T4C-containing oligonucleotides complexed with the UV-DRP were separated from the unbound oligonucleotide fraction and the frequencies of 6–4 photoproducts in the two DNA populations were compared. The UV-DRP-bound fraction was highly enriched for the 6–4 lesion over the unbound fraction supporting the conclusion that 6–4 photoproducts are the principal binding cues for the UV-DRP.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Q. Zhao, B. M. Barakat, S. Qin, A. Ray, M. A. El-Mahdy, G. Wani, E.-S. Arafa, S. N. Mir, Q.-E. Wang, and A. A. Wani The p38 Mitogen-activated Protein Kinase Augments Nucleotide Excision Repair by Mediating DDB2 Degradation and Chromatin Relaxation J. Biol. Chem., November 21, 2008; 283(47): 32553 - 32561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sugasawa Xeroderma pigmentosum genes: functions inside and outside DNA repair Carcinogenesis, March 1, 2008; 29(3): 455 - 465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Luijsterburg, J. Goedhart, J. Moser, H. Kool, B. Geverts, A. B. Houtsmuller, L. H. F. Mullenders, W. Vermeulen, and R. van Driel Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC J. Cell Sci., August 1, 2007; 120(15): 2706 - 2716. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. El-Mahdy, Q. Zhu, Q.-e. Wang, G. Wani, M. Praetorius-Ibba, and A. A. Wani Cullin 4A-mediated Proteolysis of DDB2 Protein at DNA Damage Sites Regulates in Vivo Lesion Recognition by XPC J. Biol. Chem., May 12, 2006; 281(19): 13404 - 13411. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Nishi, Y. Okuda, E. Watanabe, T. Mori, S. Iwai, C. Masutani, K. Sugasawa, and F. Hanaoka Centrin 2 Stimulates Nucleotide Excision Repair by Interacting with Xeroderma Pigmentosum Group C Protein Mol. Cell. Biol., July 1, 2005; 25(13): 5664 - 5674. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Fujiwara, C. Masutani, T. Mizukoshi, J. Kondo, F. Hanaoka, and S. Iwai Characterization of DNA Recognition by the Human UV-damaged DNA-binding Protein J. Biol. Chem., July 9, 1999; 274(28): 20027 - 20033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. Hwang, J. M. Ford, P. C. Hanawalt, and G. Chu Expression of the p48 xeroderma pigmentosum gene is p53-dependent and is involved in global genomic repair PNAS, January 19, 1999; 96(2): 424 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Hwang, S. Toering, U. Francke, and G. Chu p48 Activates a UV-Damaged-DNA Binding Factor and Is Defective in Xeroderma Pigmentosum Group E Cells That Lack Binding Activity Mol. Cell. Biol., July 1, 1998; 18(7): 4391 - 4399. [Abstract] [Full Text]
|
|
|
|
|
|
V. Otrin, I. Kuraoka, T. Nardo, M. McLenigan, A. P. M. Eker, M. Stefanini, A. S. Levine, and R. D. Wood Relationship of the Xeroderma Pigmentosum Group E DNA Repair Defect to the Chromatin and DNA Binding Proteins UV-DDB and Replication Protein A Mol. Cell. Biol., June 1, 1998; 18(6): 3182 - 3190. [Abstract] [Full Text]
|
|
|
|
|
|
V. Otrin, M McLenigan, M Takao, A. Levine, and M Protic Translocation of a UV-damaged DNA binding protein into a tight association with chromatin after treatment of mammalian cells with UV light J. Cell Sci., January 5, 1997; 110(10): 1159 - 1168. [Abstract] [PDF]
|
|