Skip Navigation

This Article
Right arrow Print PDF (681K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Wu, H.-N.
Right arrow Articles by Huang, Z.-S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, H.-N.
Right arrow Articles by Huang, Z.-S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 22 5937-5941
© 1992

MOLECULAR BIOLOGY

Mutagenesis analysis of the self-cleavage domain of hepatitis delta virus antigenomic RNA

Huey-Nan Wu and Zhi-Shun Huang

Institute of Molecular Biology, Academia Sinica Nankang, Taipei, Taiwan 11529, Republic of China

Received September 4, 1992. Revised October 26, 1992. Accepted October 26, 1992.

To determine the sequence requirements and structural features of the self-cleavage domain of hepatitis delta virus (HDV) antigenomic RNA, we constructed a series of mutants and measured the rate constant of the cleavage reaction for each. The self-cleavage activity of HDV RNA of antigenomic sense was found to reside in a region of less than 90 nucleotides in length. The catalytic domain contained a long complementary sequence which could be deleted to half of its original size. Moreover, this region could be replaced by other sequences as long as they could fold into a stem-and-loop structure. The catalytic domain also required a 6-basepair helix adjacent to the cleaving point for activity. The structural features of these two basepairing regions are quite similar to those of the HDV genomic self-cleavage domain. The cleavage site as well as the the hinge region (the sequence between the two stems) requires specific sequences for activity.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
H. Schurer, K. Lang, J. Schuster, and M. Morl
A universal method to produce in vitro transcripts with homogeneous 3' ends
Nucleic Acids Res., June 15, 2002; 30(12): e56 - e56.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Ananvoranich and J.-P. Perreault
Substrate Specificity of delta  Ribozyme Cleavage
J. Biol. Chem., May 22, 1998; 273(21): 13182 - 13188.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.