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Nucleic Acids Research, 1992, Vol. 20, No. 23 6143-6151
© 1992

SURVEY AND SUMMARY

Genome-related datasets within the E.coli Genetic Stock Center database

Mary B. Berlyn and Stanley Letovsky1

Department of Biology, Yale University New Haven, CT 06511, USA 1Letovsky Associates 268 West Rock Avenue, New Haven, CT 06515, USA

Received May 18, 1992. Revised October 19, 1992. Accepted October 19, 1992.

The contents of the E.coli Genetic Stock Center database and the availability in electronic form of the subset of information most relevant to sequence databases are described. The database uses the longstanding Stock Center records (developed and curated by Dr B.J.Bachmann) in describing genotypes of mutant derivatives of E.coli K-12 in terms of alleles, structural mutations, mating type, and plasmids as well as the derivation, names and originators of the strain, and references. The database includes descriptions of mutations, mutation properties, genes, gene properties, and gene products, with EC number identifiers for enzymes. Sequence information is not included, but entries refer to sequence database accession numbers for sequenced regions. A gene is described as a subtype of a more general category of chromosome interval called Site. Since sites are used to describe any chromosomal interval, mapping information is associated with sites. Alleles are described as mutations of those sites and they are not primary map objects, but inherit map position information from the corresponding site description. The database design is intended to preserve richness of detail where it is known and uncertainty of measurements or information as it occurs in order to represent the stock center records as accurately as possible.


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