Skip Navigation

This Article
Right arrow Print PDF (5854K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (27)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Montecucco, A.
Right arrow Articles by Ciarrocchi, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Montecucco, A.
Right arrow Articles by Ciarrocchi, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 23 6209-6214
© 1992

MOLECULAR BIOLOGY

DNA ligase I gene expression during differentiation and cell proliferation

Alessandra Montecucco, Giuseppe Biamonti, Elena Savini, Federico Focher, Silvio Spadari and Giovanni Ciarrocchi*

Istituto di Genetica Biochimica ed Evoluzionistica CNR 27100 Pavia, Italy

*To whom correspondence should be addressed

Received October 1, 1992. Revised November 3, 1992. Accepted November 3, 1992.

We have studied the regulation of mammalian DNA ligase I gene by using a cDNA probe in Northern blot experiments with RNA extracted from several cell types in different growth conditions. DNA ligase I mRNA is detected in all analysed cell systems, regardless of their proliferation state, including mature rat neurons. A significant increase in DNA ligase I mRNA level is observed when cells are induced to proliferate, in agreement with the raise of DNA joining activity found in the same cell systems. The increase parallels the start of DNA synthesis, but the messenger remains at high level beyond the end of the S fase and is detected also in the presence of aphidicolin. A decrease in DNA ligase I mRNA is observed in HL-60 and NIH-3T3 cells after differentiation. The high stability of DNA ligase I mRNA in both resting and proliferating human fibroblasts suggests a cell proliferation dependent rate of transcription. On the other hand the presence of a basal level of DNA ligase I in nondividing cells, strongly suggests an involvement of this enzyme in DNA repair. This conclusion is supported by a threefold increase in DNA ligase I observed 24 h after UV irradiation of human confluent primary fibroblasts.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
W. Wang, L. A. Lindsey-Boltz, A. Sancar, and R. A. Bambara
Mechanism of Stimulation of Human DNA Ligase I by the Rad9-Rad1-Hus1 Checkpoint Complex
J. Biol. Chem., July 28, 2006; 281(30): 20865 - 20872.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. Harrison, A.-M. Ketchen, N. J. Redhead, M. J. O'Sullivan, and D. W. Melton
Replication Failure, Genome Instability, and Increased Cancer Susceptibility in Mice with a Point Mutation in the DNA Ligase I Gene
Cancer Res., July 15, 2002; 62(14): 4065 - 4074.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
D. Sun, R. Urrabaz, S. Kelly, M. Nguyen, and S. Weitman
Enhancement of DNA Ligase I Level by Gemcitabine in Human Cancer Cells
Clin. Cancer Res., April 1, 2002; 8(4): 1189 - 1195.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
D. Sun, R. Urrabaz, M. Nguyen, J. Marty, S. Stringer, E. Cruz, L. Medina-Gundrum, and S. Weitman
Elevated Expression of DNA Ligase I in Human Cancers
Clin. Cancer Res., December 1, 2001; 7(12): 4143 - 4148.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Mossi, E. Ferrari, and U. Hubscher
DNA Ligase I Selectively Affects DNA Synthesis by DNA Polymerases delta  and epsilon  Suggesting Differential Functions in DNA Replication and Repair
J. Biol. Chem., June 5, 1998; 273(23): 14322 - 14330.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Tom, L. A. Henricksen, M. S. Park, and R. A. Bambara
DNA Ligase I and Proliferating Cell Nuclear Antigen Form a Functional Complex
J. Biol. Chem., June 29, 2001; 276(27): 24817 - 24825.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.