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Nucleic Acids Research, 1992, Vol. 20, No. 23 6261-6266
© 1992

MOLECULAR BIOLOGY

HIV-1 promotor insertion revealed by selective detection of chimeric provirus-host gene transcripts

Ines Raineri and Hans-Peter Senn*

Institute for Medical Microbiology, University of Basel Petersplatz 10, 4003 Basel, Switzerland

*To whom correspondence should be addressed

Received September 9, 1992. Revised November 6, 1992. Accepted November 6, 1992.

To study host gene activation by retroviral promotor insertion, a polymerase chain reaction (PCR) assay was developed. This method allows a sensitive and selective detection of chimeric provirus-host gene transcripts, hallmarks of insertional activation events, which does not rely on an induction of tumor cell growth. We analysed HIV-1 infected cells of a CD4+ T-cell line (H9), infected peripheral blood mononuclear cells and cells in broncho-alveolar washes of AIDS patients. In each case, a variety of chimeric mRNA molecules were detected using a PCR amplification reaction and 5' primers specific to the HIV-1 LTR and 3' primers specific to poly A of mRNA. In infected H9 lymphocytes, a mRNA was identified encoding a putative protein of 145 amino-acids that was not expressed in uninfected H9 cells. This shows for the first time that HIV-1 can activate transcription of host cellular genes by promotor insertion in a fashion similar to slow-transforming avian and munne retroviruses.


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