Nucleic Acids Research, 1992, Vol. 20, No. 24 6451-6454
© 1992
MOLECULAR BIOLOGY |
The TATA-binding protein participates in TFIIIB assembly on tRNA genes
DBCM—Service de Biochimie et Génétique Moléculaire, Centre d'Etudes de Saclay F91191 Gif-sur-Yvette cedex, France
*To whom correspondence should be addressed
Received November 4, 1992. Revised November 17, 1992. Accepted November 17, 1992.
The TATA-binding protein TBP has been recently recognized as a general class III transcription factor. Using the gel shift assay to monitor initiation complex assembly on a yeast tRNA gene, we show that TBP is required for the TFIIIC-dependent assembly of TFIIIB. TFIIIB depleted of TBP by a simple chromatographic step does not bind stably to the TFIIIC-tDNA complex. Addition of yeast or human recombinant TBP allows the formation of a TFIIIB-TBP-TFIIIC-tDNA complex. The presence of TBP in the complex was inferred from the effect of anti-TBP antibodies and from the different migration properties of TFIIIB-TBP-tDNA complexes formed with yeast or human TBP.
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