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Nucleic Acids Research, 1992, Vol. 20, No. 24 6565-6573
© 1992

CHEMISTRY

Solid-phase synthesis of branched oligoribonucleotides related to messenger RNA splicing intermediates

Masad J. Damha*, Kanjana Ganeshan, Robert H.E. Hudson and Steven V. Zabarylo

Department of Chemistry, Erindale College, University of Toronto Mississauga, Ontario L5L 1C6, Canada

*To whom correspondence should be addressed at: Department of Chemistry, McGill University, Montreal, P.Q., H3A 2K6, Canada

Received September 17, 1992. Revised October 20, 1992. Accepted October 20, 1992.

The chemical synthesis of oligoribonucleotides containing vicinal (2'-5')- and (3'-5')- phosphodiester linkages is described. The soiid-phase method, based on silyl-phosphoramidite chemistry, was applied to the synthesis of a seiles of branched RNA [(Xp)nA2' (pN)n3'(pN)n] reiated to the splicing intermediates derived from Saccharomyces cerevisiae rp51a premessenger RNA. The branched oligonucleotides have been thoroughiy characterized by nucleoside and branched nucleotide composition analysis. Branched oligoribonucieotides will be useful in the study of messenger RNA splicing and in determining the biological role of RNA ‘lariats’ and ‘forks’ in vivo.


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