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Nucleic Acids Research, 1992, Vol. 20, No. 3 445-447
© 1992

MOLECULAR BIOLOGY

Imaging and nanodissection of individual supercoiled plasmids by atomic force microscopy

Eric Henderson

Department of Zoology and Genetics, Iowa State University Ames, IA 50011, USA

Received November 12, 1991. Revised December 17, 1991. Accepted December 17, 1991.

The atomic force microscope (AFM) was used to image supercooled plasmid DMA deposited on a mica surface in either a hydrated or desiccated state. Hydrated plasmid was precisely cut by the scanning tip at a location determined by the instrument operator. Small pieces of DNA (100–150 nm in length) were excised and deposited adjacent to the dissected plasmid, demonstrating that it is possible to remove and manipulate genomic DNA fragments, unresolvable by light microscopy, from defined chromosomal locations by AFM.


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