Skip Navigation

This Article
Right arrow Print PDF (3849K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (29)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Peakman, T. C.
Right arrow Articles by Gewert, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peakman, T. C.
Right arrow Articles by Gewert, D. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 3 495-500
© 1992

MOLECULAR BIOLOGY

Highly efficient generation of recombinant baculoviruses by enzymatically mediated site-specific in vitro recombination

Timothy C. Peakman, Robert A. Harris and Dirk R. Gewert

Department of Cell Biology, Wellcome Research Laboratories South Eden Park Road, Beckenham, Kent BR3 3BS, UK

Received October 23, 1991. Revised February 7, 1992. Accepted February 7, 1992.

We have used the Cre-lox system of bacteriophage P1 to develop a highly efficient in vitrosystem for construction of recombinant baculoviruses. A positive visual selection has been included to make identification of recombinant viral progeny rapid and straightforward. We report recombination frequencies as high as 5x107 recombinants/µg starting plasmid DNA and under certain conditions, up to 50% of the viral progeny are recombinants. Genes inserted into the baculovirus genome can be readily recovered in a simple one step process and re-inserted after manipulation if required. We have confirmed the structure of recovered plasmids by diagnostic restriction endonuclease digestion and the structure of recombinant viral genomes by Southern analysis. Possible uses and the significance of the system are discussed and experiments currently being done to improve it are described.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
K. J. Airenne, E. Peltomaa, V. P. Hytonen, O. H. Laitinen, and S. Yla-Herttuala
Improved generation of recombinant baculovirus genomes in Escherichia coli
Nucleic Acids Res., September 1, 2003; 31(17): e101 - e101.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
J. L. Hartley, G. F. Temple, and M. A. Brasch
DNA Cloning Using In Vitro Site-Specific Recombination
Genome Res., November 1, 2000; 10(11): 1788 - 1795.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.