Nucleic Acids Research, 1992, Vol. 20, No. 3 513-520
© 1992
MOLECULAR BIOLOGY |
In vitro squelching of activated transcription by serum response factor: evidence for a common coactivator used by muliple transcriptional activators
Department of Biological Sciences, Columbia University New York, NY 10027, USA
Received October 4, 1991. Revised February 2, 1992. Accepted February 2, 1992.
Low amounts of serum response factor (SRF) activate transcription in vitro from a fos promoter construct containing an SRF binding site. Using this human HeLa cell-derived in vitro transcription system, we have found that high amounts of SRF inhibited, or ‘squelched’, transcription from this construct. Transcription from several other promoters activated by different gene-specific factors, including CREB and the acidic activator VP16, was also inhibited by high amounts of SRF. Basal transcription, from TATA-only promoters, however, was not inhibited. These results suggest that SRF binds to a common factor(s) (termed coactivator) required for activated transcription by a diverse group of transcriptional activators. Inhibition of transcription by SRF could be blocked by a double stranded oligonucleotide containing an SRF binding site. Mutations in SRF which abolished its DNA binding activity also reduced its ability to inhibit transcription. In addition, a C-terminal truncation of SRF which reduced its ability to activate transcription also reduced SRF's ability to inhibit transcription. These results suggest that activation and inhibition of transcription may be mediated by SRF binding to the same factor and that SRF can only bind to this factor when SRF is bound to plasmid DNA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Lin, J. McGrath, P. Wang, and T. Lee Cellular Toxicity Induced by SRF-Mediated Transcriptional Squelching Toxicol. Sci., March 1, 2007; 96(1): 83 - 91. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hayashi, S. Nakamura, W. Nishida, and K. Sobue Bone Morphogenetic Protein-Induced Msx1 and Msx2 Inhibit Myocardin-Dependent Smooth Muscle Gene Transcription Mol. Cell. Biol., December 15, 2006; 26(24): 9456 - 9470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhe, Y. Yang, and L. Schuger Imbalanced Plasminogen System in Lymphangioleiomyomatosis: Potential Role of Serum Response Factor Am. J. Respir. Cell Mol. Biol., January 1, 2005; 32(1): 28 - 34. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. K. Hogarth, N. Sandbo, S. Taurin, V. Kolenko, J. M. Miano, and N. O. Dulin Dual role of PKA in phenotypic modulation of vascular smooth muscle cells by extracellular ATP Am J Physiol Cell Physiol, August 1, 2004; 287(2): C449 - C456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Chang, L. Wei, T. Otani, K. A. Youker, M. L. Entman, and R. J. Schwartz Inhibitory Cardiac Transcription Factor, SRF-N, Is Generated by Caspase 3 Cleavage in Human Heart Failure and Attenuated by Ventricular Unloading Circulation, July 29, 2003; 108(4): 407 - 413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Osborne, H. Zhang, W.-M. Yang, E. Seto, and G. Blanck Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation Mol. Cell. Biol., October 1, 2001; 21(19): 6495 - 6506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Morin, P. Paradis, A. Aries, and M. Nemer Serum Response Factor-GATA Ternary Complex Required for Nuclear Signaling by a G-Protein-Coupled Receptor Mol. Cell. Biol., February 15, 2001; 21(4): 1036 - 1044. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. M. Miano, M. J. Carlson, J. A. Spencer, and R. P. Misra Serum Response Factor-dependent Regulation of the Smooth Muscle Calponin Gene J. Biol. Chem., March 24, 2000; 275(13): 9814 - 9822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Morris, N. Jafari, A. C. Rice, O. Vasconcelos, and R. J. DeLorenzo Persistent Increased DNA-Binding and Expression of Serum Response Factor Occur with Epilepsy-Associated Long-Term Plasticity Changes J. Neurosci., October 1, 1999; 19(19): 8234 - 8243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Spencer, M. H. Baron, and E. N. Olson Cooperative Transcriptional Activation by Serum Response Factor and the High Mobility Group Protein SSRP1 J. Biol. Chem., May 28, 1999; 274(22): 15686 - 15693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kannan and M. A. Tainsky Coactivator PC4 Mediates AP-2 Transcriptional Activity and Suppresses ras-Induced Transformation Dependent on AP-2 Transcriptional Interference Mol. Cell. Biol., January 1, 1999; 19(1): 899 - 908. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nevels, S. Rubenwolf, T. Spruss, H. Wolf, and T. Dobner The adenovirus E4orf6 protein can promote E1A/E1B-induced focus formation by interfering with p53 tumor suppressor function PNAS, February 18, 1997; 94(4): 1206 - 1211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gupta, P. Kogut, F. J. Davis, N. S. Belaguli, R. J. Schwartz, and M. P. Gupta Physical Interaction between the MADS Box of Serum Response Factor and the TEA/ATTS DNA-binding Domain of Transcription Enhancer Factor-1 J. Biol. Chem., March 23, 2001; 276(13): 10413 - 10422. [Abstract] [Full Text] [PDF]
|
|