Nucleic Acids Research, 1992, Vol. 20, No. 3 595-600
© 1992
MOLECULAR BIOLOGY |
Sequence specific generation of a DNA panhardle permits PCR amplication of unknown flanking DNA
Department of Pediatrics, University of lowa College of Medicine lowa City, IA 52242, USA
Received June 4, 1991. Revised December 20, 1991. Accepted December 20, 1991.
We present a novel method for the PCR amplification of unknown DMA that flanks a known segment directly from human genomic DMA. PCR requires that primer annealing sites be present on each end of the DNA segment that is to be amplified. In this method, known DNA is placed on the uncharacterized side of the sequence of interest via DNA polymerase mediated generation of a PCR template that is shaped like a pan with a handle. Generation of this template permits specific amplication of the unknown sequence. Tag (DNA) polymerase was used to form the one template and to generate the PCR product. 2.2 kb of the ß-globin gene, and 657 bp of the 5' flanking region of the cystic fibrosis transmembrane conductance regulator gene, were amplified directly from human genomic DNA using primers that initially flank only one side of the region amplified. This method will provide a powerful tool for acquiring ONA sequence information.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Jiang, J. Pei, X. Song, and W. Shao Restriction Site-dependent PCR: An Efficient Technique for Fast Cloning of New Genes of Microorganisms DNA Res, December 17, 2007; (2007) dsm023v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wang, J. He, Z. Cui, and S. Li Self-Formed Adaptor PCR: a Simple and Efficient Method for Chromosome Walking Appl. Envir. Microbiol., August 1, 2007; 73(15): 5048 - 5051. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Mueller, D. McDougald, D. Cusumano, N. Sodhi, S. Kjelleberg, F. Azam, and D. H. Bartlett Vibrio cholerae Strains Possess Multiple Strategies for Abiotic and Biotic Surface Colonization J. Bacteriol., July 15, 2007; 189(14): 5348 - 5360. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Lim and K. S. J. Elenitoba-Johnson Mass Spectrometry-based Proteomic Studies of Human Anaplastic Large Cell Lymphoma Mol. Cell. Proteomics, October 1, 2006; 5(10): 1787 - 1798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. J. Elenitoba-Johnson, D. K. Crockett, J. A. Schumacher, S. D. Jenson, C. M. Coffin, A. L. Rockwood, and M. S. Lim Proteomic identification of oncogenic chromosomal translocation partners encoding chimeric anaplastic lymphoma kinase fusion proteins PNAS, May 9, 2006; 103(19): 7402 - 7407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Tan, Y. Gao, M. Shi, X. Zhang, S. He, Z. Chen, and C. An SiteFinding-PCR: a simple and efficient PCR method for chromosome walking Nucleic Acids Res., August 2, 2005; 33(13): e122 - e122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ryu, K. Lee, H.-J. Hawng, C.-K. Yoo, W.-K. Seong, and H.-B. Oh Molecular Characterization of Korean Bacillus anthracis Isolates by Amplified Fragment Length Polymorphism Analysis and Multilocus Variable-Number Tandem Repeat Analysis Appl. Envir. Microbiol., August 1, 2005; 71(8): 4664 - 4671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Meyer, B. Schneider, M. Reichel, S. Angermueller, S. Strehl, S. Schnittger, C. Schoch, M. W. J. C. Jansen, J. J. van Dongen, R. Pieters, et al. Diagnostic tool for the identification of MLL rearrangements including unknown partner genes PNAS, January 11, 2005; 102(2): 449 - 454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yuanxin, A. Chengcai, L. Li, G. Jiayu, T. Guihong, and C. Zhangliang T-linker-specific ligation PCR (T-linker PCR): an advanced PCR technique for chromosome walking or for isolation of tagged DNA ends Nucleic Acids Res., June 15, 2003; 31(12): e68 - e68. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kurahashi, T. H. Shaikh, and B. S. Emanuel Alu-mediated PCR artifacts and the constitutional t(11;22) breakpoint Hum. Mol. Genet., November 1, 2000; 9(18): 2727 - 2732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Megonigal, E. F. Rappaport, R. B. Wilson, D. H. Jones, J. A. Whitlock, J. A. Ortega, D. J. Slater, P. C. Nowell, and C. A. Felix Panhandle PCR for cDNA: A rapid method for isolation of MLL fusion transcripts involving unknown partner genes PNAS, July 30, 2000; (2000) 150241797. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Sakamoto, H. Gouzu, K. Komiya, D. Kiga, S. Yokoyama, T. Yokomori, and M. Hagiya Molecular Computation by DNA Hairpin Formation Science, May 19, 2000; 288(5469): 1223 - 1226. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. A. Felix, C. S. Kim, M. D. Megonigal, D. J. Slater, D. H. Jones, N. B. Spinner, T. Stump, M. R. Hosler, P. C. Nowell, B. J. Lange, et al. Panhandle Polymerase Chain Reaction Amplifies MLL Genomic Translocation Breakpoint Involving Unknown Partner Gene Blood, December 15, 1997; 90(12): 4679 - 4686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D H Jones Panhandle PCR. Genome Res., April 1, 1995; 4(5): S195 - S201. [PDF]
|
|
|
|
|
|
M J Allen, A Collick, and A J Jeffreys Use of vectorette and subvectorette PCR to isolate transgene flanking DNA. Genome Res., October 1, 1994; 4(2): 71 - 75. [Abstract] [PDF]
|
|
|
|
|
|
D H Jones and S C Winistorfer Genome walking with 2- to 4-kb steps using panhandle PCR. Genome Res., February 1, 1993; 2(3): 197 - 203. [Abstract] [PDF]
|
|
|
|
|
|
M. D. Megonigal, E. F. Rappaport, R. B. Wilson, D. H. Jones, J. A. Whitlock, J. A. Ortega, D. J. Slater, P. C. Nowell, and C. A. Felix Panhandle PCR for cDNA: A rapid method for isolation of MLL fusion transcripts involving unknown partner genes PNAS, August 15, 2000; 97(17): 9597 - 9602. [Abstract] [Full Text] [PDF]
|
|