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Nucleic Acids Research, 1992, Vol. 20, No. 4 665-669
© 1992

Articles

Differential expression of genes encoding the hypusine-containing translation initiation factor, elF-5A, in tobacco

Danuta Chamot and Cris Kuhlemeier*

Institute of Plant Physiology, University of Berne, Altenbergrain 21 CH-3013, Berne, Switzerland

*To whom correspondence should be addressed

Received December 20, 1991. Revised January 23, 1992. Accepted January 23, 1992.

Two Nicotiana plumbaginifolia cDNA clones, NelF-5A1 and NelF-5A2, encoding eukaryotlc translation initiation factor elF-5A (formerly called elF-4D) were cloned by heterologous screening with Dictyostellum and human elF-5A probes. elF-5A Is the only protein known to contain a unique amlno acid modification, hypusine. Comparison of the Nicotiana deduced amino acid sequences with those of other elF-5A polypeptldes reveals conservation throughout the coding sequence, especially In the region of the hypusine residue. Transcript analysis reveals that NelF-5A1 is preferentially expressed in photosynthetlc tissues, while NelF-5A2 is constitutively expressed in all plant tissues examined. A polyclonal antibody was raised against NelF-5A1 overexpressed in E. coll. NelF-5A1 antiserum crossreacts with an 18 kDa polypeptide doublet In all tobacco tissues examined. At least one polypeptide of ca. 18 kDa from a diversity of higher and lower plants crossreacts with NelF-5A1 antiserum.


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