Skip Navigation

This Article
Right arrow Print PDF (6485K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (51)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by FIinn, H. M.
Right arrow Articles by Smith, D. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by FIinn, H. M.
Right arrow Articles by Smith, D. F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 4 755-762
© 1992

Articles

Genomic organisation and expression of a differentially-regulated gene family from Leishmania major

Helen M. FIinn and Deborah F. Smith*

Department of Biochemistry, Imperial College of Science, Technology and Medicine London SW7 2AZ, UK

*To whom correspondence should be addressed

Received November 22, 1991. Revised January 24, 1992. Accepted January 24, 1992.

We have isolated and characterised a differentially-regulated gene family In the protozoan parasite Leishmanla major. The family contains 5 genes linked within a 10Kb region of the genome: three of the genes are closely related in DNA sequence, the other two have only limited homology. Post-transcrlptlonal control of the differential expression pattern is suggested by detection of precursor RNA molecules containing Intergenic sequences and evidence that mature mRNA molecules contain a 35nt spliced leader sequence at their 5' ends. These features support a model of polyclstronlc transcription in which the stability and differential processing of precursor RNA molecules determine the steady state levels of mature mRNA. We have identified several DNA sequence motifs within the gene family that have potential roles in differential processing and/or RNA stability: an alternative 5' splice acceptor site for trans-splicing; a putative polyadenylation site; and a region of potential secondary structure within 3' flanking sequences. The 3' sequence elements are conserved In those genes that share the same pattern of differential regulation. To our knowledge, this Is the first example of coordinated differential-regulation of a non-identical gene cluster In Leishmania.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
N. Boucher, Y. Wu, C. Dumas, M. Dube, D. Sereno, M. Breton, and B. Papadopoulou
A Common Mechanism of Stage-regulated Gene Expression in Leishmania Mediated by a Conserved 3'-Untranslated Region Element
J. Biol. Chem., May 24, 2002; 277(22): 19511 - 19520.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Abuin, L. H. G. Freitas-Junior, W. Colli, M. J. M. Alves, and S. Schenkman
Expression of trans-Sialidase and 85-kDa Glycoprotein Genes in Trypanosoma cruzi Is Differentially Regulated at the Post-transcriptional Level by Labile Protein Factors
J. Biol. Chem., May 7, 1999; 274(19): 13041 - 13047.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Lamontagne and B. Papadopoulou
Developmental Regulation of Spliced Leader RNA Gene in Leishmania donovani Amastigotes Is Mediated by Specific Polyadenylation
J. Biol. Chem., March 5, 1999; 274(10): 6602 - 6609.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
A. C. Ivens, S. M. Lewis, A. Bagherzadeh, L. Zhang, H. M. Chan, and D. F. Smith
A Physical Map of the Leishmania major Friedlin Genome
Genome Res., February 1, 1998; 8(2): 135 - 145.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
H. Charest, W.-W. Zhang, and G. Matlashewski
The Developmental Expression of Leishmania donovani A2 Amastigote-specific Genes Is Post-transcriptionally Mediated and Involves Elements Located in the 3'-Untranslated Region
J. Biol. Chem., July 19, 1996; 271(29): 17081 - 17090.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
J H LeBowitz, H Q Smith, L Rusche, and S M Beverley
Coupling of poly(A) site selection and trans-splicing in Leishmania.
Genes & Dev., June 1, 1993; 7(6): 996 - 1007.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.