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Nucleic Acids Research, 1992, Vol. 20, No. 4 825-829
© 1992

Articles

Glucocorticoid induction of CRE-binding protein isoform mRNAs in rat C6 glioma cells

Richard A. Jungmann, Xue-song Wang, Deborah M. Milkowski and Marc L. Short

Department of Cellular, Molecular, and Structural Biology and Cancer Center, Northwestern University Medical School Chicago, IL 60611, USA

Received October 30, 1991. Revised January 21, 1992. Accepted January 21, 1992.

Mammalian cells express several distinct isoforms of transcription factor CREB (cAMP-responsive element binding protein). At least two forms, {alpha}- and {Delta}CREB, arise through alternative splicing of the CREB gene transcript. In this communication we demonstrate that the mRNAs of several CREB isoforms are expressed in rat C6 glioma cells and that the Intracellular levels of these mRNAs are markedly induced by the synthetic glucocorticoid dexamethasone. Nuclear run-off assays show that the induction occurs, at least in part, through a transcriptlonal mechanism. The enhanced cellular levels of CREB mRNAs are accompanied by increased CREB protein and CRE-binding activity of nuclear extracts as evaluated by immunoblot and Southwestern blot assays.


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