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Nucleic Acids Research, 1992, Vol. 20, No. 4 847-850
© 1992

Articles

Processing in the 5' region of the pnp transcript facilitates the site-specific endonucleolytic cleavages of mRNA

Renkichi Takata, Mika Izuhara1 and Koichi Akiyama

Department of Biotechnology, College of Agriculture, Ehime University 3-5-7 Tarumi, Matsuyama,Ehime 790 1Department of Biology, Saga Medical School Nabeshima, Saga 849, Japan

Received October 16, 1991. Revised January 21, 1992. Accepted January 21, 1992.

The primary transcript of pnp, the gene encoding polynucleotide phosphorylase in Escherichia coli, is processed in the 5' end region by ribonuclease III (RNase III). The unprocessed transcript shows enhanced stability compared with the processed transcript. We report here that, unlike the processed transcript, the unprocessed pnp transcript did not accept endonucleolytic attack at, at least, five cleavage sites. Sequencing analysis of the four cleavage products shows no sequence specific to all these sites, but AU rich stretches were observed at three sites.


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