Nucleic Acids Research, 1992, Vol. 20, No. 5 1053-1059
© 1992
MOLECULAR BIOLOGY |
Assignment of a yeast protein necessary for mitochondrial transcription initiation
Department of Developmental Biology, Stanford University School of Medicine Stanford, CA 94305-5427, USA
*To whom correspondence should be addressed
Received December 9, 1991. Revised January 24, 1992. Accepted January 24, 1992.
Yeast mitochondrial DNA contains multiple promoters that are responsible for expression of its genes. The basic yeast mitochondrial promoter consists of a nonanucleotide consensus sequence [5'-ATATAA-GTA( +1)-3'] that must be recognized by transcription proteins, including mitochondrial RNA polymerase and any relevant trans-acting factors. Since mitochondrial RNA polymerase alone appeared unable to recognize a mitochondrlal promoter, we examined the effects of providing accessory proteins to enable promoter function. After expression in Escherichia coli or purification from yeast mitochondria, two proteins were tested; they were ABF2 (a structural homologue of the human mitochondrial transcriptlonal activator mtTF1) and MTF1 (the gene product of a yeast locus known to exhibit a mitochondrial transcription phenotype). The results show that MTF1 specifies correct transcriptional initiation while ABF2 does not. We conclude that MTF1 is an essential key protein in yeast mitochondrial promoter function. Considering the increasing complexity of the mitochondrial transcription apparatus, we propose a nomenclature system for its components.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. C. Scarpulla Transcriptional Paradigms in Mammalian Mitochondrial Biogenesis and Function Physiol Rev, April 1, 2008; 88(2): 611 - 638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. E. Shutt and M. W. Gray Homologs of Mitochondrial Transcription Factor B, Sparsely Distributed Within the Eukaryotic Radiation, Are Likely Derived from the Dimethyladenosine Methyltransferase of the Mitochondrial Endosymbiont Mol. Biol. Evol., June 1, 2006; 23(6): 1169 - 1179. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Matsushima, R. Garesse, and L. S. Kaguni Drosophila Mitochondrial Transcription Factor B2 Regulates Mitochondrial DNA Copy Number and Transcription in Schneider Cells J. Biol. Chem., June 25, 2004; 279(26): 26900 - 26905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. F. Bogenhagen, Y. Wang, E. L. Shen, and R. Kobayashi Protein Components of Mitochondrial DNA Nucleoids in Higher Eukaryotes Mol. Cell. Proteomics, November 1, 2003; 2(11): 1205 - 1216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. McCulloch and G. S. Shadel Human Mitochondrial Transcription Factor B1 Interacts with the C-Terminal Activation Region of h-mtTFA and Stimulates Transcription Independently of Its RNA Methyltransferase Activity Mol. Cell. Biol., August 15, 2003; 23(16): 5816 - 5824. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Lisowsky, D. Wilkens, T. Stein, B. Hedtke, T. Borner, and A. Weihe The C-terminal Region of Mitochondrial Single-subunit RNA Polymerases Contains Species-specific Determinants for Maintenance of Intact Mitochondrial Genomes Mol. Biol. Cell, July 1, 2002; 13(7): 2245 - 2255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. McCulloch, B. L. Seidel-Rogol, and G. S. Shadel A Human Mitochondrial Transcription Factor Is Related to RNA Adenine Methyltransferases and Binds S-Adenosylmethionine Mol. Cell. Biol., February 15, 2002; 22(4): 1116 - 1125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. L. Shen and D. F. Bogenhagen Developmentally-regulated packaging of mitochondrial DNA by the HMG-box protein mtTFA during Xenopus oogenesis Nucleic Acids Res., July 1, 2001; 29(13): 2822 - 2828. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wang and G. S. Shadel Stability of the mitochondrial genome requires an amino-terminal domain of yeast mitochondrial RNA polymerase PNAS, July 6, 1999; 96(14): 8046 - 8051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. F. Bogenhagen and D. F. Bogenhagen Interaction of mtTFB and mtRNA Polymerase at Core Promoters for Transcription of Xenopus laevis mtDNA J. Biol. Chem., May 17, 1996; 271(20): 12036 - 12041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Eriksson, B. Xu, and D. A. Clayton Efficient Incorporation of Anti-HIV Deoxynucleotides by Recombinant Yeast Mitochondrial DNA Polymerase J. Biol. Chem., August 11, 1995; 270(32): 18929 - 18934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C A Virbasius, J V Virbasius, and R C Scarpulla NRF-1, an activator involved in nuclear-mitochondrial interactions, utilizes a new DNA-binding domain conserved in a family of developmental regulators. Genes & Dev., December 1, 1993; 7(12a): 2431 - 2445. [Abstract] [PDF]
|
|
|
|
|
|
M. S. Rodeheffer, B. E. Boone, A. C. Bryan, and G. S. Shadel Nam1p, a Protein Involved in RNA Processing and Translation, Is Coupled to Transcription through an Interaction with Yeast Mitochondrial RNA Polymerase J. Biol. Chem., March 9, 2001; 276(11): 8616 - 8622. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kanazawa, Y. Nishio, A. Kashiwagi, H. Inagaki, R. Kikkawa, and K. Horiike Reduced activity of mtTFA decreases the transcription in mitochondria isolated from diabetic rat heart Am J Physiol Endocrinol Metab, April 1, 2002; 282(4): E778 - E785. [Abstract] [Full Text] [PDF]
|
|