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Nucleic Acids Research, 1992, Vol. 20, No. 5 1109-1112
© 1992

CHEMISTRY

Conformational transitions of poly(dA-bromo5dU) and poly(dA-iodo5dU) in solution

Michaela Vorlickovaá, Jana Chlaádkovaá and Jaroslav Kypr

Institute of Biophysics, Czechoslovak Academy of Sciences CS-61265 Brno, Czechoslovakia

Received November 8, 1991. Revised January 20, 1992. Accepted January 20, 1992.

Extensive circular dichroism studies have been conducted with the title polynucleotides under various solution conditions. The studies provided the following information: (i) The halogen atoms in place of thymine methyl hinder the Isomerization into X-DNA. (ii) The brominated but not iodinated polynudeotide isomerizes into Z-DNA in concentrated NaCI + NiCl2- The transition takes place at lower NiCl2 concentrations than with poty(dA-dT). (iii) The iodinated polynucleotide forms an unusual conformation in aqueous solution in which it is very stable, it isomerizes from this conformer into the usual B-type double helix in concentrated ethanol solutions. The isomerization is a two-state cooperative process, (iv) Both title polynucleotides undergo still another two-state cooperative transition in trifluorethanol solutions presumably into A-DNA showing a rather unusual circular dichroism spectrum.


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