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Nucleic Acids Research, 1992, Vol. 20, No. 5 975-982
© 1992

MOLECULAR BIOLOGY

The initiation accuracy of the SV40 early transcription is determined by the functional domains of two TATA elements

Marc Pauly, Micheèle Treger1, Eric Westhof1 and Pierre Chambon2

Laboratoire de Recherche sur le Cancer et les Maladies du Sang, Zone industrielle Grasbusch, L-3370 Leudelange, Luxembourg 1Laboratoire de Cristallographie Biologique du CNRS, Institut de Biologie Moleéculaire et Cellulaire rue Reneé Descartes 15, F-67084 Strasbourg Ceédex 2Laboratoire de Geéneétique Moleéculaire des Eucaryotes du CNRS/ Unité 184 de Biologie MolÉculaire et de Geénieé Geéneétique de I'lNSERM, Institut de Chimie Biologique, Faculteé de Meédecine rue Humann 11, F-67085 Strasbourg Ceédex, France

Received January 16, 1992. Accepted January 30, 1992.

To locate the boundaries of the TATA element in the SV40 early promoter, point mutations have been constructed such as to cover the whole T + A-rich region of the replication origin. The effects of these mutations on the rate of transcription in vivo show that this region actually contains two TATA elements I and II, each Independently directing the accurate initiation of transcription from a specified set of start sites, EES1 and EES2, respectively. The sequence of TATA element I fits best with the compiled ‘consensus’ sequence found in eukaryotic gene promoters and is the most efficient in directing transcription initiation. Mutations which improve this fit can still increase the rate of transcription, confirming the theory of a correlation between the nucleotide sequence of a TATA element and its functional efficiency. Moreover, some mutations which simultaneously modify the angle of DNA curvature in the T + A-rich promoter region and the rate of transcription reveal a correlation between DNA bending and transcription initiation.


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