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Nucleic Acids Research, 1992, Vol. 20, No. 6 1265-1269
© 1992

CHEMISTRY

Chemical synthesis of RNA using fast oligonucleotide deprotection chemistry

Ravi Vinayak, Pamela Anderson1, Christie McCollum and Arnold Hampel1

Applied Biosystems Inc., 850 Lincoln Centre Drive Foster City, CA 94404 , USA 1Department of Biological Sciences, Northern Illinois University DeKalb, IL 60115-28611, USA

Received January 3, 1992. Revised February 13, 1992. Accepted February 13, 1992.

The exocyclic amine protecting groups in oligonucleotide synthesis which require 8–16 hours at 55°C for deprotection in ammonia have been replaced with more labile base protecting groups (dimethylformamidine for adenine and guanine and isobutyryl for cytoslne). Using these fast oligonucleotide deprotecting groups which require 2–3 hours at 55°C for complete deprotection, a new set of cyanoethyl phosphoramidite rlbonucleoside monomers and supports has been developed. Ribozymes and substrate RNAs which were synthesized with these phosphoramidites were assayed and were found to have full catalytic (biological) activity.


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