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Nucleic Acids Research, 1992, Vol. 20, No. 6 1283-1287
© 1992

MOLECULAR BIOLOGY

The 4-nitroquinoline 1-oxide mutational spectrum in single stranded DNA is characterized by guanine to pyrimidine transversions

Gilberto Fronza1, Paola Campomenosi1, Raffaella lannone1 and Angelo Abbondandolo1,2

1Laboratory of Mutagenesis, National Institute for Research on Cancer (IST), Viale Benedetto XV 10,16132 Genova 2Cattedra di Genetica, University of Genoa Italy

Received December 20, 1991. Revised February 13, 1992. Accepted February 13, 1992.

4-Nitroquinoline-1 -oxide is a potent mutagen and carcinogen which induces two main guanine adducts at positions C8 and N2. In ds or ss damaged DNA the ratio C8/N2 adducts is 1:2 and 8–10:1, respectively. In bacteria and yeast 4NQO has been shown to be a base substitution mutagen acting at G residues inducing mainly G to A transitions. We determined the mutational spectrum induced by the 4NQO metabolite, acetoxy-4-aminoquinoline 1-oxide, in the M137sol;acZ7 E.coli lacZ{Delta}AM15 {alpha} complementation assay using ssDNA. Among 68 Ac-4HAQ0 induced mutants, G to Pyr transverslon was the most frequent base substitution observed. By comparison with dsDNA based systems, our data suggest that dGuo-C8-AQO induces G to Pyr transversions. A mechanism to explain how this lesion may induce transversions is proposed.


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