Nucleic Acids Research, 1992, Vol. 20, No. 6 1301-1308
© 1992
MOLECULAR BIOLOGY |
Analysis of the phosphorylation, DNA-binding and dimerization properties of the RNA polymerase I transcription factors UBF1 and UBF2
Geisinger Clinic, Sigfried and Janet Weis Center for Research 100 Academy Avenue, Danville, PA 17822-2618, USA
*To whom correspondence should be addressed
Received December 13, 1991. Revised February 7, 1992. Accepted February 7, 1992.
The phosphorylation, DNA-binding and dimerization properties of both forms of the RNA polymerase I transcription factor UBF were studied and compared. Tryptlc peptlde maps of In vivo 32P-labeled UBF contained four phospho-peptides. Two of these peptides are predicted to derive from the serine-rich, carboxyl-terminal of UBF. This region contains nine consensus phosphorylation sites for casein klnase II, and is one of the regions phosphorylated in vitro by casein kJnase II. Analysis of the DNA-binding properties of recombinant forms of UBF1 and UBF2 by Southwestern blots revealed: (1) a role for the NH2-terminal 102 amino acid domain of UBF1/UBF2 in DNA-binding; (2) the importance of the bases from –106 to –101 of the rat ribosomal DNA promoter for the binding of UBF; and (3) functional differences between UBF1 and UBF2. Glutaraldehyde cross-linking and overlay assays using recombinant forms of UBF1 and UBF2 demonstrated that the molecules can form both homodimers and heterodimers. These assays also demonstrated that the NH2-terminal 102 amino acids of UBF plays a significant role in dimerization and that other domains contribute to dimerization. The dimerization properties of recombinant forms of UBF1 and UBF2 were different, suggesting that the HMG box 2 of UBF1, which is partially deleted in UBF2, also contributes to UBF dimerization.
+Present address: Center for Cardiovascular Research, Department of Medicine and Experimental Therapeutics, University College Dublin, Mater Hospital, 41 Eccles Street, Dublin 7, Ireland
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C.-Y. Lin, S. Navarro, S. Reddy, and L. Comai CK2-mediated stimulation of Pol I transcription by stabilization of UBF-SL1 interaction Nucleic Acids Res., October 18, 2006; 34(17): 4752 - 4766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. B. Panova, K. I. Panov, J. Russell, and J. C. B. M. Zomerdijk Casein kinase 2 associates with initiation-competent RNA polymerase I and has multiple roles in ribosomal DNA transcription. Mol. Cell. Biol., August 1, 2006; 26(16): 5957 - 5968. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C. Valdez, D. Henning, R. B. So, J. Dixon, and M. J. Dixon The Treacher Collins syndrome (TCOF1) gene product is involved in ribosomal DNA gene transcription by interacting with upstream binding factor PNAS, July 20, 2004; 101(29): 10709 - 10714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Grueneberg, L. Pablo, K.-Q. Hu, P. August, Z. Weng, and J. Papkoff A Functional Screen in Human Cells Identifies UBF2 as an RNA Polymerase II Transcription Factor That Enhances the {beta}-Catenin Signaling Pathway Mol. Cell. Biol., June 1, 2003; 23(11): 3936 - 3950. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Hirschler-Laszkiewicz, A. H. Cavanaugh, A. Mirza, M. Lun, Q. Hu, T. Smink, and L. I. Rothblum Rrn3 Becomes Inactivated in the Process of Ribosomal DNA Transcription J. Biol. Chem., May 23, 2003; 278(21): 18953 - 18959. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. H. Cavanaugh, I. Hirschler-Laszkiewicz, Q. Hu, M. Dundr, T. Smink, T. Misteli, and L. I. Rothblum Rrn3 Phosphorylation Is a Regulatory Checkpoint for Ribosome Biogenesis J. Biol. Chem., July 19, 2002; 277(30): 27423 - 27432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kermekchiev and L. Ivanova Ribin, a Protein Encoded by a Message Complementary to rRNA, Modulates Ribosomal Transcription and Cell Proliferation Mol. Cell. Biol., December 15, 2001; 21(24): 8255 - 8263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fath, P. Milkereit, G. Peyroche, M. Riva, C. Carles, and H. Tschochner Differential roles of phosphorylation in the formation of transcriptional active RNA polymerase I PNAS, November 15, 2001; (2001) 231181398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Voit and I. Grummt Phosphorylation of UBF at serine 388 is required for interaction with RNA polymerase I and activation of rDNA transcription PNAS, October 31, 2001; (2001) 231071698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Y. Stefanovsky, G. Pelletier, D. P. Bazett-Jones, C. Crane-Robinson, and T. Moss DNA looping in the RNA polymerase I enhancesome is the result of non-cooperative in-phase bending by two UBF molecules Nucleic Acids Res., August 1, 2001; 29(15): 3241 - 3247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. I. Panov, J. K. Friedrich, and J. C. B. M. Zomerdijk A Step Subsequent to Preinitiation Complex Assembly at the Ribosomal RNA Gene Promoter Is Rate Limiting for Human RNA Polymerase I-Dependent Transcription Mol. Cell. Biol., April 15, 2001; 21(8): 2641 - 2649. [Abstract] [Full Text]
|
|
|
|
 |
|
 Q Tian, G. Kopf, R. Brown, and H Tseng Function of basonuclin in increasing transcription of the ribosomal RNA genes during mouse oogenesis Development, January 2, 2001; 128(3): 407 - 416. [Abstract] [PDF]
|
|
|
|
|
|
M. R. Paule and R. J. White SURVEY AND SUMMARY Transcription by RNA polymerases I and III Nucleic Acids Res., March 15, 2000; 28(6): 1283 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Klein and I. Grummt Cell cycle-dependent regulation of RNA polymerase I transcription: The nucleolar transcription factor UBF is inactive in mitosis and early G1 PNAS, May 25, 1999; 96(11): 6096 - 6101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Zhai and L. Comai A Kinase Activity Associated with Simian Virus 40 Large T Antigen Phosphorylates Upstream Binding Factor (UBF) and Promotes Formation of a Stable Initiation Complex between UBF and SL1 Mol. Cell. Biol., April 1, 1999; 19(4): 2791 - 2802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Tuan, W. Zhai, and L. Comai Recruitment of TATA-Binding Protein-TAFI Complex SL1 to the Human Ribosomal DNA Promoter Is Mediated by the Carboxy-Terminal Activation Domain of Upstream Binding Factor (UBF) and Is Regulated by UBF Phosphorylation Mol. Cell. Biol., April 1, 1999; 19(4): 2872 - 2879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-C. Albert, M. Denton, M. Kermekchiev, and C. S. Pikaard Histone Acetyltransferase and Protein Kinase Activities Copurify with a Putative Xenopus RNA Polymerase I Holoenzyme Self-Sufficient for Promoter-Dependent Transcription Mol. Cell. Biol., January 1, 1999; 19(1): 796 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Kihm, J. C. Hershey, T. A. J. Haystead, C. S. Madsen, and G. K. Owens Phosphorylation of the rRNA transcription factor upstream binding factor promotes its association with TATA binding protein PNAS, December 8, 1998; 95(25): 14816 - 14820. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Hautmann, M. M. Thompson, E. A. Swartz, E. N. Olson, and G. K. Owens Angiotensin II–Induced Stimulation of Smooth Muscle {alpha}-Actin Expression by Serum Response Factor and the Homeodomain Transcription Factor MHox Circ. Res., October 19, 1997; 81(4): 600 - 610. [Abstract] [Full Text]
|
|
|
|
 |
|
 A Kohler, M. Schmidt-Zachmann, and W. Franke AND-1, a natural chimeric DNA-binding protein, combines an HMG-box with regulatory WD-repeats J. Cell Sci., January 5, 1997; 110(9): 1051 - 1062. [Abstract] [PDF]
|
|
|
|
|
|
J. Luyken, R. D. Hannan, J. Y. Cheung, and L. I. Rothblum Regulation of rDNA Transcription During Endothelin-1Induced Hypertrophy of Neonatal Cardiomyocytes : Hyperphosphorylation of Upstream Binding Factor, an rDNA Transcription Factor Circ. Res., March 1, 1996; 78(3): 354 - 361. [Abstract] [Full Text]
|
|
|
|
|
|
R. D. Hannan, J. Luyken, and L. I. Rothblum Regulation of Ribosomal DNA Transcription during Contraction-induced Hypertrophy of Neonatal Cardiomyocytes J. Biol. Chem., February 9, 1996; 271(6): 3213 - 3220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Hershey, M. Hautmann, M. M. Thompson, L. I. Rothblum, T. A. J. Haystead, and G. K. Owens Angiotensin II-induced Hypertrophy of Rat Vascular Smooth Muscle Is Associated with Increased 18 S rRNA Synthesis and Phosphorylation of the rRNA Transcription Factor, Upstream Binding Factor J. Biol. Chem., October 20, 1995; 270(42): 25096 - 25101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Hannan, J. Luyken, and L. I. Rothblum Regulation of rDNA Transcription Factors during Cardiomyocyte Hypertrophy Induced by Adrenergic Agents J. Biol. Chem., April 7, 1995; 270(14): 8290 - 8297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y Cui, K W Hagan, S Zhang, and S W Peltz Identification and characterization of genes that are required for the accelerated degradation of mRNAs containing a premature translational termination codon. Genes & Dev., February 15, 1995; 9(4): 423 - 436. [Abstract] [PDF]
|
|
|
|
|
|
R. Voit and I. Grummt Phosphorylation of UBF at serine 388 is required for interaction with RNA polymerase I and activation of rDNA transcription PNAS, November 20, 2001; 98(24): 13631 - 13636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fath, P. Milkereit, G. Peyroche, M. Riva, C. Carles, and H. Tschochner Differential roles of phosphorylation in the formation of transcriptional active RNA polymerase I PNAS, December 4, 2001; 98(25): 14334 - 14339. [Abstract] [Full Text] [PDF]
|
|