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Nucleic Acids Research, 1992, Vol. 20, No. 6 1333-1337
© 1992

GENOME STRUCTURE AND MAPPING

3' Alu PCR: a simple and rapid method to isolate human polymorphic markers

J.-P. Chariieu, A.-M. Laurent, D.A. Carter, M. Bellis and G. Roizeès

CNRS UPR 8402 & INSERM U. 249, Institut de Biologie 34060 Montpellier cedex, France

Received November 22, 1991. Revised February 19, 1992. Accepted February 19, 1992.

Microsatellites, such as (TG)n found at random throughout the genome, or as 3' extensions of Alu sequences are being Increasingly used as genetic markers because of their pluriallelic character. The search for polymorphic microsatellites is time consuming, however, as it is necessary to sequence clones containing the microsatellites sequences in order to design specific PCR primers before testing for polymorphism, which does not always occur. We propose here a new approach to generate polymorphic markers, based on the amplification of microsatellites at the 3' end of Alu sequences, without the need for cloning or sequencing steps.


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