Skip Navigation

This Article
Right arrow Print PDF (2040K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (21)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Truss, M.
Right arrow Articles by Beato, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Truss, M.
Right arrow Articles by Beato, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 7 1483-1486
© 1992

MOLECULAR BIOLOGY

Artificial steroid hormone response element generated by dam-methylation

Mathias Truss, Jörg Bartsch, Georges Chalepakis1 and Miguel Beato*

Institut für Molekularbiologie und Tumorforschung, E.-Mannkopff-StraBe 2, D-3550 Marburg 1Max-Planck-lnsitut für Biophysikalische-Chemie, Nikolausberg, 3400 Göttingen Germany

*To whom correspondence should be addressed

Received February 11, 1992. Revised March 9, 1992. Accepted March 9, 1992.

Using the interaction of steroid hormone receptors with their pallndromic response elements as an example, we show here that cloning in dam+ bacterial strains can lead to artifactual results due to methylation of adenine residues at the N-6 position. Substitution of the T by an A in the third position of the half palindromes of the hormone responsive element TGTTCT(1) yields a functional element only when amplification is made in dam+ bacteria. Mutant palindromes methylated at the N-6 position of this adenine exhibit the same affinity for progesterone and glucocorticoid receptors as the consensus response element, whereas their unmethylated counterpart binds with negligible affinity. These observations underline the significance of hydrophobic interactions between receptors and the major groove of the DNA for discrimination among various responsive elements, and point to the importance of using dam– bacterial strains for the correct identification of the nucleotide sequence of cis-acting elements.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
M. Christian, Y. Pohnke, R. Kempf, B. Gellersen, and J. J. Brosens
Functional Association of PR and CCAAT/Enhancer-Binding Protein {beta} Isoforms: Promoter-Dependent Cooperation between PR-B and Liver-Enriched Inhibitory Protein, or Liver-Enriched Activatory Protein and PR-A in Human Endometrial Stromal Cells
Mol. Endocrinol., January 1, 2002; 16(1): 141 - 154.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Schweizer-Groyer, N. Jibard, E. Neau, D. Fortin, F. Cadepond, E.-E. Baulieu, and A. Groyer
The Glucocorticoid Response Element II Is Functionally Homologous in Rat and Human Insulin-like Growth Factor-binding Protein-1 Promoters
J. Biol. Chem., April 23, 1999; 274(17): 11679 - 11686.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
M. Boruk, J. G. A. Savory, and R. J. G. Haché
AF-2-Dependent Potentiation of CCAAT Enhancer Binding Protein {beta}-Mediated Transcriptional Activation by Glucocorticoid Receptor
Mol. Endocrinol., November 1, 1998; 12(11): 1749 - 1763.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.