Nucleic Acids Research, 1992, Vol. 20, No. 7 1539-1545
© 1992
MOLECULAR BIOLOGY |
Mammalian p53 can function as a transcription factor in yeast
Oncogene Group, Swiss Institute for Experimental Cancer Research, ISREC, Ch. des Boveresses 155 CH-1066 Epalinges, Switzerland
*To whom correspondence should be addressed
Received January 20, 1992. Revised March 11, 1992. Accepted March 11, 1992.
p53 has previously been shown to contain a transactivation domain using GAL4 fusion proteins and to bind specifically to a 33 base pair DNA sequence in immunoprecipitation assays. We show here that mammalian p53 expressed in S.cerevisiae is able to activate transcription of a reporter gene placed under the control of a CYC1 hybrid promoter containing the 33 base pair p53-blnding sequence. The activation is dependent on the orientation and number of copies of the binding site. Three p53 mutants commonly found in human tumours, 175H, 248W and 273H, are unable to activate transcription. A fourth human p53 mutant, 285K, is temperature-sensitive for transcriptional activation. Murine p53 activates transcription from the same sequence. The murine 135V mutant, which is temperature-sensitive for mammalian cell transformation, is also temperature-sensitive for transcriptional activation. There is a much better correlation between mutation and transcriptional competence than between mutation and the structure of p53 determined with conformation-sensitive antibodies. We have therefore developed a simple transcription assay for p53 mutation in which yeast are transfected with p53 PCR products and mutation is scored on X-gal plates.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. R. Dearth, H. Qian, T. Wang, T. E. Baroni, J. Zeng, S. W. Chen, S. Y. Yi, and R. K. Brachmann Inactive full-length p53 mutants lacking dominant wild-type p53 inhibition highlight loss of heterozygosity as an important aspect of p53 status in human cancers Carcinogenesis, February 1, 2007; 28(2): 289 - 298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Ellison, J. Lofing, and G. A. Bitter Human MutL homolog (MLH1) function in DNA mismatch repair: a prospective screen for missense mutations in the ATPase domain Nucleic Acids Res., October 8, 2004; 32(18): 5321 - 5338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Del Carratore, A. Petrucci, M. Simili, G. Fronza, and A. Galli Involvement of human p53 in induced intrachromosomal recombination in Saccharomyces cerevisiae Mutagenesis, September 1, 2004; 19(5): 333 - 339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Valadez and F. P. Guengerich S-(2-Chloroethyl)glutathione-generated p53 Mutation Spectra Are Influenced by Differential Repair Rates More than Sites of Initial DNA Damage J. Biol. Chem., April 2, 2004; 279(14): 13435 - 13446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Yoon, C.-S. Lee, and G. P. Pfeifer Simulated sunlight and benzo[a]pyrene diol epoxide induced mutagenesis in the human p53 gene evaluated by the yeast functional assay: lack of correspondence to tumor mutation spectra Carcinogenesis, January 1, 2003; 24(1): 113 - 119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Inga, F. Storici, T. A. Darden, and M. A. Resnick Differential Transactivation by the p53 Transcription Factor Is Highly Dependent on p53 Level and Promoter Target Sequence Mol. Cell. Biol., December 15, 2002; 22(24): 8612 - 8625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Boley, T. P. McManus, V. M. Maher, and J. J. McCormick Malignant Transformation of Human Fibroblast Cell Strain MSU-1.1 by N-Methyl-N-nitrosourea: Evidence of Elimination of p53 by Homologous Recombination Cancer Res., August 1, 2000; 60(15): 4105 - 4111. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Das, J. Bohl, and S. B. Vande Pol Identification of a Second Transforming Function in Bovine Papillomavirus Type 1 E6 and the Role of E6 Interactions with Paxillin, E6BP, and E6AP J. Virol., January 1, 2000; 74(2): 812 - 816. [Abstract] [Full Text]
|
|
|
|
|
|
D. P. Xirodimas and D. P. Lane Molecular Evolution of the Thermosensitive PAb1620 Epitope of Human p53 by DNA Shuffling J. Biol. Chem., September 24, 1999; 274(39): 28042 - 28049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liu, D. M. Scolnick, R. C. Trievel, H. B. Zhang, R. Marmorstein, T. D. Halazonetis, and S. L. Berger p53 Sites Acetylated In Vitro by PCAF and p300 Are Acetylated In Vivo in Response to DNA Damage Mol. Cell. Biol., February 1, 1999; 19(2): 1202 - 1209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. D. Pearson and G. F. Merrill Deletion of the Saccharomyces cerevisiae TRR1 Gene Encoding Thioredoxin Reductase Inhibits p53-dependent Reporter Gene Expression J. Biol. Chem., March 6, 1998; 273(10): 5431 - 5434. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Humphrey, A. Salim, M. R. Erdos, F. S. Collins, L. C. Brody, and R. D. Klausner Human BRCA1 inhibits growth in yeast: Potential use in diagnostic testing PNAS, May 27, 1997; 94(11): 5820 - 5825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Moshinsky and G. N. Wogan UV-induced mutagenesis of human p53 in a vector replicated in Saccharomyces cerevisiae PNAS, March 18, 1997; 94(6): 2266 - 2271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hansen, C. A. Midgley, D. P. Lane, B. C. Freeman, R. I. Morimoto, and T. R. Hupp Modification of Two Distinct COOH-terminal Domains Is Required for Murine p53 Activation by Bacterial Hsp70 J. Biol. Chem., November 29, 1996; 271(48): 30922 - 30928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Hupp and D. P. Lane Two Distinct Signaling Pathways Activate the Latent DNA Binding Function of p53 in a Casein Kinase II-independent Manner J. Biol. Chem., July 28, 1995; 270(30): 18165 - 18174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-S. Hsu, F.-M. Tang, W.-L. Liu, J.-Y. Chuang, M.-Y. Lai, and Y.-S. Lin Transcriptional Regulation by p53 J. Biol. Chem., March 24, 1995; 270(12): 6966 - 6974. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Milne, L. E. Campbell, D. G. Campbell, and D. W. Meek p53 Is Phosphorylated in Vitro and in Vivo by an Ultraviolet Radiation-induced Protein Kinase Characteristic of the c-Jun Kinase, JNK1 J. Biol. Chem., March 10, 1995; 270(10): 5511 - 5518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Bargonetti, J J Manfredi, X Chen, D R Marshak, and C Prives A proteolytic fragment from the central region of p53 has marked sequence-specific DNA-binding activity when generated from wild-type but not from oncogenic mutant p53 protein. Genes & Dev., December 1, 1993; 7(12b): 2565 - 2574. [Abstract] [PDF]
|
|
|
|
|
|
X Chen, G Farmer, H Zhu, R Prywes, and C Prives Cooperative DNA binding of p53 with TFIID (TBP): a possible mechanism for transcriptional activation. Genes & Dev., October 1, 1993; 7(10): 1837 - 1849. [Abstract] [PDF]
|
|
|
|
|
|
J Bargonetti, I Reynisdottir, P N Friedman, and C Prives Site-specific binding of wild-type p53 to cellular DNA is inhibited by SV40 T antigen and mutant p53. Genes & Dev., October 1, 1992; 6(10): 1886 - 1898. [Abstract] [PDF]
|
|