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Nucleic Acids Research, 1992, Vol. 20, No. 7 1559-1565
© 1992


GENOME STRUCTURE AND MAPPING

A physical and genetic map of the Spiroplasma citri genome

Fengchun Ye1, Frédéric Laigret1,*, Jane C. Whitley1,2, Christine Citti1, Lloyd R. Finch2, Patricia Carle1, Joël Renaudin1 and Joseph-Marie Bové1

1Laboratoire de Biologie cellulaire et moléculaire 33883 Villenave d'Ornon Cedex, France 2Russel Grimwade School of Biochemistry, University of Melbourne Parkwille, Victoria, Australia

*To whom correspondence should be addressed

Received January 17, 1992. Revised March 3, 1992. Accepted March 3, 1992.

A physical and genetic map of the Spiroplasma citri genome has been constructed using several restriction enzymes and pulsed field gel electrophoresis. A number of genes were subsequently localized on the map by the use of appropriate probes. The genome size of the spiroplasma estimated from restriction fragments is close to 1780 kbp, the largest of all Mollicutes studied so far. It contains multisite insertions of Spiroplasma virus 1 (SpV1) sequences. The physical and genetic map of the S. citri genome shares several features with that of other Mollicutes, especially those in the Mycoplasma mycoides cluster. This supports the finding that S. citri and these Mycoplasma spp. are phylogenetically related.


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