Skip Navigation

This Article
Right arrow Print PDF (2304K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Ohyama, T.
Right arrow Articles by Sakuma, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohyama, T.
Right arrow Articles by Sakuma, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 7 1617-1622
© 1992

MOLECULAR BIOLOGY

Alteration of the curved helical structure located in the upstream region of the ß-lactamase promoter of plasmid pUC 19 and its effect on transcription

Takashi Ohyama, Masahiko Nagumo, Yoshiko Hirota and Sadatoshi Sakuma

Section of Molecular Biology, Meiji Institute of Health Science 540 Naruda, Odawara 250, Japan

Received December 30, 1991. Revised March 6, 1992. Accepted March 6, 1992.

The region preceding the ß-lactamase promoter of Escherichia coli plasmid pUC19 has a curved DNA (bent DNA) structure. The center of the curvature was revealed to exist around nucleotide position 2580 of the plasmid, which is just beside RNA polymerase binding region. It was indicated that the identified region is curved even at 60° C. The gross geometry of the curvature was altered by inserting synthetic double-stranded oligonucleotides between positions 2585 and 2586. Effect of the alteration on strength of the promoter was not detectedin vitro. However, in vivo analyses showed that the promoter strength is apparently dependent, in part, on the gross geometry of the curvature. Insertions of 4 and 16 bp, both of which altered the gross geometry of the curvature greatly, caused considerable reductions of in vivo level of ß-lactamase mRNA. in vivo, overall three-dimensional structure of the region covering the promoter and the curvature seems to play some significant role in transcription of the gene.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
M. Asayama, H. Kato, J. Shibato, M. Shirai, and T. Ohyama
The curved DNA structure in the 5'-upstream region of the light-responsive genes: its universality, binding factor and function for cyanobacterial psbA transcription
Nucleic Acids Res., November 1, 2002; 30(21): 4658 - 4666.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Angermayr, U. Oechsner, K. Gregor, G. P. Schroth, and W. Bandlow
Transcription initiation in vivo without classical transactivators: DNA kinks flanking the core promoter of the housekeeping yeast adenylate kinase gene, AKY2, position nucleosomes and constitutively activate transcription
Nucleic Acids Res., October 1, 2002; 30(19): 4199 - 4207.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
B. Fournier, A. Gravel, D. C. Hooper, and P. H. Roy
Strength and Regulation of the Different Promoters for Chromosomal beta -Lactamases of Klebsiella oxytoca
Antimicrob. Agents Chemother., April 1, 1999; 43(4): 850 - 855.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. E. Aiyar, R. L. Gourse, and W. Ross
Upstream A-tracts increase bacterial promoter activity through interactions with the RNA polymerase alpha  subunit
PNAS, December 8, 1998; 95(25): 14652 - 14657.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Ohyama and T. Ohyama
Bent DNA in the Human Adenovirus Type 2E1A Enhancer Is an Architectural Element for Transcription Stimulation
J. Biol. Chem., November 1, 1996; 271(44): 27823 - 27828.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Basak, L. Olsen, S. Hattman, and V. Nagaraja
Intrinsic DNA Distortion of the Bacteriophage Mu momP1 Promoter Is a Negative Regulator of Its Transcription. A NOVEL MODE OF REGULATION OF TOXIC GENE EXPRESSION
J. Biol. Chem., June 1, 2001; 276(23): 19836 - 19844.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.